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Literature summary for 3.2.1.164 extracted from

  • Okemoto, K.; Uekita, T.; Tsumuraya, Y.; Hashimoto, Y.; Kasama, T.
    Purification and characterization of an endo-beta-(1-6)-galactanase from Trichoderma viride (2003), Carbohydr. Res., 338, 219-230.
    View publication on PubMed
Search for more literature for 3.2.1.164

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17000
-
 gel filtration Trichoderma viride
47000
-
 native PAGE Trichoderma viride

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme form a commercial cellulase preparation, 57fold to homogeneity by ammonium sulfate fractionation, two steps of ion exchange chromatography, and gel filtration Trichoderma viride

Reaction

Reaction Comment Organism Reaction ID
Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products mode of action, overview Trichoderma viride
a

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation commercial cellulase preparation Onozuka R-10 Trichoderma viride
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-galactan + H2O galactan from Prototheca zopfii, which has a high proportion of beta-1,6-linked galactosyl residues, the extent of hydrolysis of the carbohydrate portion of a radish root arabinogalactan-protein AGP increases when alpha-L-arabinofuranosyl residues attached to beta-1,6-linked galactosyl side chains of the AGP are removed in advance, the enzyme releases galactose, beta-1,6-galactobiose, and 4-O-methyl-beta-glucuronosyl-1,6-galactose as major hydrolysis products when allowed to act exhaustively on the modified AGP, overview Trichoderma viride ?
-
 ?
additional information substrate preparations and specificity, the endo-galactanase hydrolyzes specifically beta-1,6-galactooligosaccharides with a degree of polymerization higher than 3 and their acidic derivatives with 4-O-methyl-glucosyluronic or glucosyluronic groups at the nonreducing terminals, oveview Trichoderma viride ?
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-(1-6)-galactanase
-
 Trichoderma viride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
-
 Trichoderma viride