Cloned (Comment) | Organism |
---|---|
DNA and maino acid sequence determination and analysis, sequence comparison, expression in Escherichia coli strain BL21 as His6-tagged enzyme fused to thioredoxin | Trichoderma viride |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50488 | - |
x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
50687 | - |
x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
64000 | - |
x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
68296 | - |
x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma viride | Q76FP5 | IFO 31 137 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain Bl21 by two steps of metal chelating chromatography, the thioredoxin tag is cleaved off | Trichoderma viride |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Endohydrolysis of (1->6)-beta-D-galactosidic linkages in arabinogalactan proteins and (1->3):(1->6)-beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products | mode of action, overview | Trichoderma viride |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity, overview | Trichoderma viride | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50488, sequence calculation, x * 64000, recombinant enzyme, SDS-PAGE, x * 50687, detagged recombinant enzyme, mass spectrometry, x * 68296, recombinant His6-tagged thioredoxin fusion enzyme, sequence calculation | Trichoderma viride |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-(1-6)-galactanase | - |
Trichoderma viride |
Tv6GAL | - |
Trichoderma viride |