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Literature summary for 3.2.1.162 extracted from
- A novel enzyme, lambda-carrageenase, isolated from a deep-sea bacterium (2006), J. Biochem., 140, 475-481.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Pseudoalteromonas sp. |
General Stability
| General Stability | Organism |
|---|---|
| sorbitol, sucrose and trehalose improve stability | Pseudoalteromonas sp. |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| CsCl | maximal activity in presence of 0.4 M NaCl. CsCl is able to compensate for KCl | Pseudoalteromonas sp. |  |
| KCl | maximal activity in presence of 0.4 M NaCl. KCl is able to compensate for KCl | Pseudoalteromonas sp. | |
| LiCl | maximal activity in presence of 0.4 M NaCl. LiCl is able to compensate for KCl | Pseudoalteromonas sp. | |
| NaCl | maximal activity in presence of 0.4 M NaCl | Pseudoalteromonas sp. | |
| RbCl | maximal activity in presence of 0.4 M NaCl. RbCl is able to compensate for KCl | Pseudoalteromonas sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | - |
gel filtration | Pseudoalteromonas sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas sp. | Q05JY7 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudoalteromonas sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | containing lambda-carrageenan as a carbon source | Pseudoalteromonas sp. | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lambda-carrageenan + H2O | - |
Pseudoalteromonas sp. | alpha-D-Galp2,6S2-(1-3)-beta-D-Galp2S-(1-4)-alpha-D-Galp2,6S2-(1-3)-D-Galp2S + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 100000, SDS-PAGE | Pseudoalteromonas sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-1,4-carrageenose 2,6,2'-trisulfate-hydrolase | - |
Pseudoalteromonas sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Pseudoalteromonas sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | 20°C: about 40% of maximal activity, 40°C: about 65% of maximal activity | Pseudoalteromonas sp. |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
120 min, stable | Pseudoalteromonas sp. |
| 30 | - |
0.5 M sorbitol, 120 min, enzyme is stable up to | Pseudoalteromonas sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Pseudoalteromonas sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | pH 6.0: about 40% of maximal activity, pH 8.5: about 45% of maximal activity | Pseudoalteromonas sp. |
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Release 2023.1 (January 2023)
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