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Literature summary for 3.2.1.162 extracted from
- Degradation of lambda-carrageenan by Pseudoalteromonas carrageenovora lambda-carrageenase: a new family of glycoside hydrolases unrelated to kappa- and iota-carrageenases (2007), Biochem. J., 404, 105-114.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed as His-tag fusion protein in Escherichia coli, formation of inclusion bodies that are solved in 8 M urea | Pseudoalteromonas carrageenovora |
| expression in Escherichia coli as insoluble inclusion bodies. These inclusion bodies are purified and solubilized in 8 M urea | Pseudoalteromonas carrageenovora |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Pseudoalteromonas carrageenovora | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | - |
gel filtration | Pseudoalteromonas carrageenovora |
| 97000 | - |
SDS-PAGE | Pseudoalteromonas carrageenovora |
| 97000 | - |
1 * 97000, SDS-PAGE | Pseudoalteromonas carrageenovora |
| 105000 | - |
pre-protein, calculated from the deduced amino acid sequence | Pseudoalteromonas carrageenovora |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lambda-carrageenan + H2O | Pseudoalteromonas carrageenovora | sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan | neo-lambda-carratetraose + neo-lambda-carrahexaose | main reaction products | ? |  |
| lambda-carrageenan + H2O | Pseudoalteromonas carrageenovora ATCC 43555 | sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan | neo-lambda-carratetraose + neo-lambda-carrahexaose | main reaction products | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas carrageenovora | - |
- |
- |
| Pseudoalteromonas carrageenovora | - |
ATCC43555 | - |
| Pseudoalteromonas carrageenovora | Q0JRK4 | - |
- |
| Pseudoalteromonas carrageenovora ATCC 43555 | - |
ATCC43555 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudoalteromonas carrageenovora |
| from host strain | Pseudoalteromonas carrageenovora |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| Endohydrolysis of (1->4)-beta-linkages in the backbone of lambda-carrageenan, resulting in the tetrasaccharide alpha-D-Gal-p2,6S2-(1->3)-beta-D-Gal-p2S-(1->4)-alpha-D-Gal-p2,6S2-(1->3)-D-Gal-p2-S | proceeds according to an endolytic mode of action and a mechanism of inversion of the anomeric configuration | Pseudoalteromonas carrageenovora |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Pseudoalteromonas carrageenovora | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| lambda-carrageenan + H2O | - |
Pseudoalteromonas carrageenovora | neo-lambda-carratetraose + neo-lambda-carrahexaose | main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer | ? | |
| lambda-carrageenan + H2O | CglA hydrolase proceeds according to an endolytic mode of action and a mechanism of inversion of the anomeric configuration | Pseudoalteromonas carrageenovora | neo-lambda-carratetraose + neo-lambda-carrahexaose | neo-lambda-carratetraose is the main degradation product | ? | |
| lambda-carrageenan + H2O | sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan | Pseudoalteromonas carrageenovora | neo-lambda-carratetraose + neo-lambda-carrahexaose | main reaction products | ? | |
| lambda-carrageenan + H2O | - |
Pseudoalteromonas carrageenovora ATCC 43555 | neo-lambda-carratetraose + neo-lambda-carrahexaose | main reaction products, after prolonged incubation (22-50 h) increasing ratio of neo-lambda-carratetraose suggesting also the rise of a dimeric product arising from the cleavage of the hexamer | ? | |
| lambda-carrageenan + H2O | sole known depolymerizing enzyme of lambda-carrageenan, hydrolyses the beta(1-4) linkage of lambda-carrageenan | Pseudoalteromonas carrageenovora ATCC 43555 | neo-lambda-carratetraose + neo-lambda-carrahexaose | main reaction products | ? | |
| neo-lambda-carrahexaose + H2O | - |
Pseudoalteromonas carrageenovora | neo-lambda-carratetraose + neo-lambda-carrabiose | - |
? | |
| neo-lambda-carrahexaose + H2O | - |
Pseudoalteromonas carrageenovora ATCC 43555 | neo-lambda-carratetraose + neo-lambda-carrabiose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 97000, SDS-PAGE | Pseudoalteromonas carrageenovora |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CglA | - |
Pseudoalteromonas carrageenovora |
| CglA hydrolase | - |
Pseudoalteromonas carrageenovora |
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