Literature summary for 3.2.1.161 extracted from

Chuankhayan, P.; Hua, Y.; Svasti, J.; Sakdarat, S.; Sullivan, P.A.; Ketudat Cairns, J.R.
Purification of an isoflavonoid 7-O-beta-apiosyl-glucoside beta-glycosidase and its substrates from Dalbergia nigrescens Kurz (2005), Phytochemistry, 66, 1880-1889.

Search for more literature for 3.2.1.161

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	EDTA has no effect on the activity	Dalbergia nigrescens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.09	-	genistin	-	Dalbergia nigrescens
0.11	-	daidzin	-	Dalbergia nigrescens
0.5	-	dalpatein-7-O-[beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranoside]	-	Dalbergia nigrescens
0.7	-	dalpaniculin-7-O-[beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranoside]	-	Dalbergia nigrescens
1.8	-	4-nitrophenyl-beta-D-fucoside	-	Dalbergia nigrescens
14.7	-	4-nitrophenyl-beta-D-glucopyranoside	-	Dalbergia nigrescens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	FeSO4, CaCl2, HgCl2, MnCl2, ZnSO4 have no effect on the activity	Dalbergia nigrescens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
240000	-	gel filtration	Dalbergia nigrescens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
daidzin + H2O	Dalbergia nigrescens	-	daidzein + beta-D-glucopyranose	-	ir
dalcochinin-8'-O-beta-D-glucopyranoside + H2O	Dalbergia nigrescens	-	dalcochinin + beta-D-glucopyranose	-	ir
dalpaniculin-7-O-[beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranoside] + H2O	Dalbergia nigrescens	-	dalpaniculin + beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose	-	ir
dalpatein-7-O-[beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranoside] + H2O	Dalbergia nigrescens	-	dalpatein + beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose	-	ir
genistin + H2O	Dalbergia nigrescens	-	genistein + beta-D-glucopyranose	-	ir

Organism

Organism	UniProt	Comment	Textmining
Dalbergia nigrescens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE anion exchange chromatograpy, gel filtration	Dalbergia nigrescens

Source Tissue

Source Tissue	Comment	Organism	Textmining
seed	-	Dalbergia nigrescens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
9.2	-	-	Dalbergia nigrescens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-fucoside + H2O	-	Dalbergia nigrescens	4-nitrophenol + beta-D-fucose	-	ir
4-nitrophenyl beta-D-galactopyranoside + H2O	-	Dalbergia nigrescens	4-nitrophenol + beta-D-galactopyranose	-	ir
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Dalbergia nigrescens	4-nitrophenol + D-glucopyranose	-	ir
4-nitrophenyl beta-D-mannopyranoside + H2O	-	Dalbergia nigrescens	4-nitrophenol + beta-D-mannopyranose	-	ir
4-nitrophenyl beta-D-thioglucopyranoside + H2O	-	Dalbergia nigrescens	4-nitrophenol + beta-D-thioglucopyranose	-	ir
4-nitrophenyl beta-D-xylopyranoside + H2O	-	Dalbergia nigrescens	4-nitrophenol + beta-D-xylopyranose	-	ir
4-nitrophenyl-alpha-L-arabinopyranoside + H2O	-	Dalbergia nigrescens	4-nitrophenol + alpha-L-arabinopyranose	-	ir
daidzin + H2O	-	Dalbergia nigrescens	daidzein + beta-D-glucopyranose	-	ir
dalcochinin-8'-O-beta-D-glucopyranoside + H2O	-	Dalbergia nigrescens	dalcochinin + beta-D-glucopyranose	-	ir
dalpaniculin-7-O-[beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranoside] + H2O	-	Dalbergia nigrescens	dalpaniculin + beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose	-	ir
dalpatein-7-O-[beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranoside] + H2O	-	Dalbergia nigrescens	dalpatein + beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranose	-	ir
genistin + H2O	-	Dalbergia nigrescens	genistein + beta-D-glucopyranose	-	ir

Subunits

Subunits	Comment	Organism
tetramer	4 * 62000-63000, SDS-PAGE	Dalbergia nigrescens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Dalbergia nigrescens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
7	-	4-nitrophenyl-beta-D-fucoside	-	Dalbergia nigrescens
10.4	-	4-nitrophenyl-beta-D-glucopyranoside	-	Dalbergia nigrescens
334	-	dalpaniculin-7-O-[beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranoside]	-	Dalbergia nigrescens
465	-	dalpatein-7-O-[beta-D-apiofuranosyl-(1-6)-beta-D-glucopyranoside]	-	Dalbergia nigrescens
480	-	daidzin	-	Dalbergia nigrescens
500	-	genistin	-	Dalbergia nigrescens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	6	-	Dalbergia nigrescens

pH Range

pH Minimum	pH Maximum	Comment	Organism
3.5	6.5	less than half-maximal activity above and below	Dalbergia nigrescens

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Release 2023.1 (January 2023)