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Literature summary for 3.2.1.158 extracted from

  • Liu, J.; Liu, Z.; Jiang, C.; Mao, X.
    Biochemical characterization and substrate degradation mode of a novel beta-agarase from Catenovulum agarivorans (2019), J. Agric. Food Chem., 67, 10373-10379.
    View publication on PubMed
Search for more literature for 3.2.1.158

Application

Application Comment Organism
synthesis the enzyme (CaLJ96) has potential for the manufacture of bioactive agarooligosaccharides Catenovulum agarivorans

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Catenovulum agarivorans

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ 10 mM, inhibition to 96.5% of control Catenovulum agarivorans
Co2+ 10 mM, inhibition to 28.9% of control Catenovulum agarivorans
Cu2+ 10 mM, inhibition to 3.3% of control Catenovulum agarivorans
Fe3+ 10 mM, inhibition to 1.2% of control Catenovulum agarivorans
K+ 10 mM, inhibition to 58.4% of control Catenovulum agarivorans
Mg2+ 10 mM, inhibition to 46.0% of control Catenovulum agarivorans
Mn2+ 10 mM, inhibition to 97.8% of control Catenovulum agarivorans
Na+ 10 mM, inhibition to 58.1% of control Catenovulum agarivorans
Na2EDTA 10 mM, complete inhibition Catenovulum agarivorans
Ni2+ 10 mM, complete inhibition Catenovulum agarivorans
SDS 10 mM, inhibition to 11.2% of control Catenovulum agarivorans
Zn2+ 10 mM, complete inhibition Catenovulum agarivorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 agarose KM: 0.135 mg/ml, pH 7.0, 37°C Catenovulum agarivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10 mM, activation to 116.7% of control Catenovulum agarivorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
 SDS-PAGE Catenovulum agarivorans

Organism

Organism UniProt Comment Textmining
Catenovulum agarivorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Catenovulum agarivorans

Storage Stability

Storage Stability Organism
4°C, 28 d, more than 70% of initial activity is retained Catenovulum agarivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
agarohexaose + H2O
-
 Catenovulum agarivorans agarobiose + agarotetraose
-
 ?
agaropentaose + H2O
-
 Catenovulum agarivorans agarobiose + agarotriose
-
 ?
agarose + H2O agarotetraose is the dominant product. No actiovity with: agarobiose, agarotriose and agarotetraose Catenovulum agarivorans agarobiose + agarotetraose + agarohexaose
-
 ?

Synonyms

Synonyms Comment Organism
CaLJ96
-
 Catenovulum agarivorans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Catenovulum agarivorans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 more than 80% of the highest enzyme activity is maintained at 30-50°C, and the activity at 20°C is 12.3% of that at 37°C Catenovulum agarivorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 30 1 h, stable Catenovulum agarivorans
37
-
 1 h, complete loss of activity at temperature higher than 37°C Catenovulum agarivorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
 agarose pH 7.0, 37°C Catenovulum agarivorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Catenovulum agarivorans

pH Range

pH Minimum pH Maximum Comment Organism
3 5 the activity is less than 10% of that at pH 7.0 Catenovulum agarivorans
7 10 more than 80% of the highest enzyme activity is retained at pH 7.0-10.0 Catenovulum agarivorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
 agarose kcat/Km: 17.78 ml/mg*s, pH 7.0, 37°C Catenovulum agarivorans