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Literature summary for 3.2.1.156 extracted from

  • Motomitsu, K.; Yuji, H.; Shinya, F.; Masafumi, H.; Takane, K.; Kenji, Y.
    Conversion of inverting glycoside hydrolases into catalysts for synthesizing glycosides employing a glycosynthase strategy (2009), Trends Glycosci. Glycotechnol., 21, 23-39.
No PubMed abstract available
Search for more literature for 3.2.1.156

Protein Variants

Protein Variants Comment Organism
D263C saturation mutagenesis, the mutant accumulates high amounts of xylotriose, but reduced F- releasing activity compared to the wild-type enzyme Halalkalibacterium halodurans
D263X construction of a D263 mutant library by saturation mutagenesis Halalkalibacterium halodurans
additional information method for construction of a mutant engineered reducing-end xylose-releasing exooligoxylanase, with mutation of residues Y198 and D263, that acts as a glycosynthase, reaction mechanism and modelling, overview. Glycosynthases are engineered glycoside hydrolases, that catalyze the synthesis of glycoside from glycosyl-fluoride donors and suitable acceptors, overview Halalkalibacterium halodurans
Y198F saturation mutagenesis, the mutant shows highly reduced hydrolytic activity and slightly increased F- releasing activity from alpha-xylobiosyl fluoride in presence of xylose compared to the wild-type enzyme Halalkalibacterium halodurans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-O-fluoro-4-O-alpha-D-xylopyranosyl-beta-D-xylopyranose + H2O Halalkalibacterium halodurans
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 alpha-D-xylopyranose + 1-O-fluoro-beta-D-xylopyranose
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 ?
additional information Halalkalibacterium halodurans Rex is an inverting xylanolytic enzyme belonging to the glycohydrolase family 8, GH8, hydrolyzing xylooligosacchrides to release xylose from its reducing end ?
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 ?

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-fluoro-4-O-alpha-D-xylopyranosyl-beta-D-xylopyranose + H2O
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 Halalkalibacterium halodurans alpha-D-xylopyranose + 1-O-fluoro-beta-D-xylopyranose
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 ?
1-O-fluoro-4-O-alpha-D-xylopyranosyl-beta-D-xylopyranose + H2O Rex is an inverting glycohydrolase, that shows the Hehre resynthesis-hydrolysis mechanism, overview. Residue Y198 forms a hydrogen bond with nucleophilic water Halalkalibacterium halodurans alpha-D-xylopyranose + 1-O-fluoro-beta-D-xylopyranose
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 ?
additional information Rex is an inverting xylanolytic enzyme belonging to the glycohydrolase family 8, GH8, hydrolyzing xylooligosacchrides to release xylose from its reducing end Halalkalibacterium halodurans ?
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 ?

Synonyms

Synonyms Comment Organism
More Rex is an inverting xylanolytic enzyme belonging to the glycohydrolase family 8, GH8 Halalkalibacterium halodurans
reducing-end xylose-releasing exo-oligoxylanase
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 Halalkalibacterium halodurans
Rex
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 Halalkalibacterium halodurans