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Literature summary for 3.2.1.156 extracted from
- The first glycosynthase derived from an inverting glycoside hydrolase (2006), J. Biol. Chem., 281, 1426-1431.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D263A | conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose | Halalkalibacterium halodurans |
| D263C | conversion of glycosynthase from converting enzyme, synthesis of xylotriose from xylobiose + HF + xylose, highest glycosynthase activity of mutants tested | Halalkalibacterium halodurans |
| D263G | conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose | Halalkalibacterium halodurans |
| D263L | conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose | Halalkalibacterium halodurans |
| D263N | conversion of glycosynthase from converting enzyme, synthesis of xylotriose from xylobiose + HF + xylose, second highest glycosynthase activity of mutants tested | Halalkalibacterium halodurans |
| D263P | very little glycosynthase or converting activity | Halalkalibacterium halodurans |
| D263S | very little glycosynthase or converting activity | Halalkalibacterium halodurans |
| D263T | conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose | Halalkalibacterium halodurans |
| D263V | conversion of glycosynthase from converting enzyme, synthesis of some xylotriose from xylobiose + HF + xylose | Halalkalibacterium halodurans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Halalkalibacterium halodurans | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-xylobiosylfluoride + H2O + xylose | - |
Halalkalibacterium halodurans | alpha-xylobiose + HF + xylose | presence of xylose is required, some mutants in D263 also produce xylotriose | ? |  |
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