Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Halalkalibacterium halodurans |
Protein Variants | Comment | Organism |
---|---|---|
D128A | specific activity is 290fold lower than that of the wild-type enzyme | Halalkalibacterium halodurans |
D263A | specific activity is 4421fold lower than that of the wild-type enzyme | Halalkalibacterium halodurans |
D70A | specific activity is 1545fold lower than that of the wild-type enzyme | Halalkalibacterium halodurans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | - |
Halalkalibacterium halodurans | |
0.0044 | - |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | - |
Halalkalibacterium halodurans | |
0.005 | - |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | - |
Halalkalibacterium halodurans | |
0.0185 | - |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | - |
Halalkalibacterium halodurans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Halalkalibacterium halodurans | 5622 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Halalkalibacterium halodurans | the enzyme may play a role as a key enzyme in intracellular xylan metabolism by cleaving xylooligosaccharides that are produced by the action of other intracellular enzymes from the arabino/glucurono-xylooligosaccharides | ? | - |
? | |
additional information | Halalkalibacterium halodurans C-125 | the enzyme may play a role as a key enzyme in intracellular xylan metabolism by cleaving xylooligosaccharides that are produced by the action of other intracellular enzymes from the arabino/glucurono-xylooligosaccharides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halalkalibacterium halodurans | - |
C-125 | - |
Halalkalibacterium halodurans C-125 | - |
C-125 | - |
Purification (Comment) | Organism |
---|---|
- |
Halalkalibacterium halodurans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-D-glucopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O | 1.1% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | Halalkalibacterium halodurans | ? | - |
? | |
beta-D-glucopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O | 1.1% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | Halalkalibacterium halodurans C-125 | ? | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-deoxyxylopyranose + H2O | 3.2% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | Halalkalibacterium halodurans | ? | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-deoxyxylopyranose + H2O | 3.2% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | Halalkalibacterium halodurans C-125 | ? | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-glucopyranose + H2O | 0.5% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | Halalkalibacterium halodurans | ? | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-glucopyranose + H2O | 0.5% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | Halalkalibacterium halodurans C-125 | ? | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O | the enzyme acts rapidly on the beta-anomer of beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose, leaving the new reducing end in the alpha configuration. It also acts on longer oligosaccharides that have this structure at their reducing ends. The penulimate residue must be xylose, but replacing either of the other two residues with glucose merely slows the rate greatly | Halalkalibacterium halodurans | beta-D-xylopyranosyl-(1-4)-alpha-D-xylopyranose | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O | the enzyme acts rapidly on the beta-anomer of beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose, leaving the new reducing end in the alpha configuration. It also acts on longer oligosaccharides that have this structure at their reducing ends. The penulimate residue must be xylose, but replacing either of the other two residues with glucose merely slows the rate greatly | Halalkalibacterium halodurans C-125 | beta-D-xylopyranosyl-(1-4)-alpha-D-xylopyranose | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O | - |
Halalkalibacterium halodurans | ? | - |
? | |
additional information | the enzyme may play a role as a key enzyme in intracellular xylan metabolism by cleaving xylooligosaccharides that are produced by the action of other intracellular enzymes from the arabino/glucurono-xylooligosaccharides | Halalkalibacterium halodurans | ? | - |
? | |
additional information | the enzyme may play a role as a key enzyme in intracellular xylan metabolism by cleaving xylooligosaccharides that are produced by the action of other intracellular enzymes from the arabino/glucurono-xylooligosaccharides | Halalkalibacterium halodurans C-125 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
reducing end xylose-releasing exo-oligoxylanase | - |
Halalkalibacterium halodurans |
Rex | - |
Halalkalibacterium halodurans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Halalkalibacterium halodurans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 55 | about 50% of maximal activity at 35°C and at 55°C | Halalkalibacterium halodurans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
pH 7.1, 30 min, stable at temperatures up to | Halalkalibacterium halodurans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
73 | - |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | - |
Halalkalibacterium halodurans | |
162 | - |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | - |
Halalkalibacterium halodurans | |
163 | - |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | - |
Halalkalibacterium halodurans | |
175 | - |
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose | - |
Halalkalibacterium halodurans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | 7.3 | - |
Halalkalibacterium halodurans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.3 | 8.2 | pH 5.3: about 60% of maximal activity, pH 8.2: about 50% of maximal activity | Halalkalibacterium halodurans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.8 | 30°C, 30 min, stable | Halalkalibacterium halodurans |