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Literature summary for 3.2.1.156 extracted from

  • Honda, Y.; Kitaoka, M.
    A family 8 glycoside hydrolase from Bacillus halodurans C-125 (BH2105) is a reducing end xylose-releasing exo-oligoxylanase (2004), J. Biol. Chem., 279, 55097-55103.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Halalkalibacterium halodurans

Protein Variants

Protein Variants Comment Organism
D128A specific activity is 290fold lower than that of the wild-type enzyme Halalkalibacterium halodurans
D263A specific activity is 4421fold lower than that of the wild-type enzyme Halalkalibacterium halodurans
D70A specific activity is 1545fold lower than that of the wild-type enzyme Halalkalibacterium halodurans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
Halalkalibacterium halodurans
0.0044
-
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
Halalkalibacterium halodurans
0.005
-
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
Halalkalibacterium halodurans
0.0185
-
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
Halalkalibacterium halodurans

Localization

Localization Comment Organism GeneOntology No. Textmining
intracellular
-
Halalkalibacterium halodurans 5622
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Halalkalibacterium halodurans the enzyme may play a role as a key enzyme in intracellular xylan metabolism by cleaving xylooligosaccharides that are produced by the action of other intracellular enzymes from the arabino/glucurono-xylooligosaccharides ?
-
?
additional information Halalkalibacterium halodurans C-125 the enzyme may play a role as a key enzyme in intracellular xylan metabolism by cleaving xylooligosaccharides that are produced by the action of other intracellular enzymes from the arabino/glucurono-xylooligosaccharides ?
-
?

Organism

Organism UniProt Comment Textmining
Halalkalibacterium halodurans
-
C-125
-
Halalkalibacterium halodurans C-125
-
C-125
-

Purification (Commentary)

Purification (Comment) Organism
-
Halalkalibacterium halodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-glucopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O 1.1% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose Halalkalibacterium halodurans ?
-
?
beta-D-glucopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O 1.1% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose Halalkalibacterium halodurans C-125 ?
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-deoxyxylopyranose + H2O 3.2% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose Halalkalibacterium halodurans ?
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-deoxyxylopyranose + H2O 3.2% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose Halalkalibacterium halodurans C-125 ?
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-glucopyranose + H2O 0.5% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose Halalkalibacterium halodurans ?
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-glucopyranose + H2O 0.5% of the activity with beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose Halalkalibacterium halodurans C-125 ?
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O the enzyme acts rapidly on the beta-anomer of beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose, leaving the new reducing end in the alpha configuration. It also acts on longer oligosaccharides that have this structure at their reducing ends. The penulimate residue must be xylose, but replacing either of the other two residues with glucose merely slows the rate greatly Halalkalibacterium halodurans beta-D-xylopyranosyl-(1-4)-alpha-D-xylopyranose
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O the enzyme acts rapidly on the beta-anomer of beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose, leaving the new reducing end in the alpha configuration. It also acts on longer oligosaccharides that have this structure at their reducing ends. The penulimate residue must be xylose, but replacing either of the other two residues with glucose merely slows the rate greatly Halalkalibacterium halodurans C-125 beta-D-xylopyranosyl-(1-4)-alpha-D-xylopyranose
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O
-
Halalkalibacterium halodurans ?
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O
-
Halalkalibacterium halodurans ?
-
?
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O
-
Halalkalibacterium halodurans ?
-
?
additional information the enzyme may play a role as a key enzyme in intracellular xylan metabolism by cleaving xylooligosaccharides that are produced by the action of other intracellular enzymes from the arabino/glucurono-xylooligosaccharides Halalkalibacterium halodurans ?
-
?
additional information the enzyme may play a role as a key enzyme in intracellular xylan metabolism by cleaving xylooligosaccharides that are produced by the action of other intracellular enzymes from the arabino/glucurono-xylooligosaccharides Halalkalibacterium halodurans C-125 ?
-
?

Synonyms

Synonyms Comment Organism
reducing end xylose-releasing exo-oligoxylanase
-
Halalkalibacterium halodurans
Rex
-
Halalkalibacterium halodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Halalkalibacterium halodurans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 55 about 50% of maximal activity at 35°C and at 55°C Halalkalibacterium halodurans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
pH 7.1, 30 min, stable at temperatures up to Halalkalibacterium halodurans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
73
-
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
Halalkalibacterium halodurans
162
-
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
Halalkalibacterium halodurans
163
-
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
Halalkalibacterium halodurans
175
-
beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
Halalkalibacterium halodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2 7.3
-
Halalkalibacterium halodurans

pH Range

pH Minimum pH Maximum Comment Organism
5.3 8.2 pH 5.3: about 60% of maximal activity, pH 8.2: about 50% of maximal activity Halalkalibacterium halodurans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 9.8 30°C, 30 min, stable Halalkalibacterium halodurans