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Literature summary for 3.2.1.155 extracted from
- Two new major subunits in the cellulosome of Clostridium thermocellum: xyloglucanase Xgh74A and endoxylanase Xyn10D (2005), Microbiology, 151, 3395-3401.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cellulosome | - |
Acetivibrio thermocellus | 43263 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 81800 | - |
- |
Acetivibrio thermocellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | Acetivibrio thermocellus | Xgh74 contributes to the in vivo degradation of the hemicellulose xyloglucan and xylan by the cellulosome of Clostridium thermocellum | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
| Acetivibrio thermocellus | Q70DK5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-glucan + H2O | 8% of the activity with xyloglucan | Acetivibrio thermocellus | beta-glucan oligosaccharides | - |
? | |
| carboxymethyl cellulose + H2O | 5% of the activity with xyloglucan | Acetivibrio thermocellus | carboxymethyl cellulose oligosaccharides | - |
? | |
| additional information | low activity on carboxymethyl cellulose and on glucuronoxylan. Lack of activity on amorphous cellulose | Acetivibrio thermocellus | ? | - |
? | |
| xyloglucan + H2O | Xgh74 contributes to the in vivo degradation of the hemicellulose xyloglucan and xylan by the cellulosome of Clostridium thermocellum | Acetivibrio thermocellus | ? | - |
? | |
| xyloglucan + H2O | the enzyme is a true endo-glucanase when it acts on linear substrates without bulky substituents in the polymer backbone. When the enzyme acts on polymeric substrate with bulky side chains, its mode of action is switched to exo-like | Acetivibrio thermocellus | xyloglucan oligosaccharides | XXXG, XXLG and XLLG oligosaccharides | ? | |
| xyloglucan + H2O | tamarind xyloglucan. Xgh74A hydrolyzes every fourth beta-1,4-glucan bond in the xyloglucan backbone, thus producing decorated cellotetraose units | Acetivibrio thermocellus | cellotetraose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| xyloglucanase Xgh74A | - |
Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Acetivibrio thermocellus |
| 75 | - |
hydrolysis of tamarind seed xyloglucan | Acetivibrio thermocellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
stable up to | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.4 | - |
hydrolysis of tamarid xyloglucan | Acetivibrio thermocellus |
| 6.4 | - |
hydrolysis of tamarind seed xyloglucan | Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.7 | 7.8 | 50% of maximal activity at pH 5.7 and at pH 7.8 | Acetivibrio thermocellus |
| 5.7 | 7.8 | more than 50% of maximal activity in the pH-range 5.7-7.8, hydrolysis of tamarind seed xyloglucan | Acetivibrio thermocellus |
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Release 2023.1 (January 2023)
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