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Literature summary for 3.2.1.155 extracted from

  • Yaoi, K.; Nakai, T.; Kameda, Y.; Hiyoshi, A.; Mitsuishi, Y.
    Cloning and characterization of two xyloglucanases from Paenibacillus sp. strain KM21 (2005), Appl. Environ. Microbiol., 71, 7670-7678.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.155

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information KM-value for xyloglucan is 1.2 mg/ml Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
105000
-
 x * 105000, SDS-PAGE Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp. Q3MUH7
-
-
Paenibacillus sp. KM21 Q3MUH7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan Paenibacillus sp. ?
-
 ?
additional information no activity towards Avicel, carboxymethylcellulose, barley beta-1,3/1,4-glucan or xylan Paenibacillus sp. KM21 ?
-
 ?
XXXGXXXG + H2O
-
 Paenibacillus sp. XXX + XXXG + GXXXG
-
 ?
xyloglucan + H2O XEG74 has dual endo-mode and exo-mode activities Paenibacillus sp. xyloglucan oligosaccharides
-
 ?
xyloglucan + H2O XEG74 has dual endo-mode and exo-mode activities Paenibacillus sp. KM21 xyloglucan oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 105000, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
XEG74
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60 70
-
 Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable up to Paenibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3
-
 xyloglucan
-
 Paenibacillus sp.
77.6
-
 xyloglucan
-
 Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.5 stable Paenibacillus sp.