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Literature summary for 3.2.1.154 extracted from

  • Lothier, J.; Van Laere, A.; Prudhomme, M.P.; Van den Ende, W.; Morvan-Bertrand, A.
    Cloning and characterization of a novel fructan 6-exohydrolase strongly inhibited by sucrose in Lolium perenne (2014), Planta, 240, 629-643.
    View publication on PubMed
Search for more literature for 3.2.1.154

Cloned(Commentary)

Cloned (Comment) Organism
cDNA library screening, gene Lp6-FEHa, DNA and amino acid sequence determination and analysis, phylogenetic analysis Lolium perenne

Inhibitors

Inhibitors Comment Organism Structure
sucrose the enzyme is 70-90% inhibited by 10-40 mM sucrose Lolium perenne

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6,6-kestotetraose + H2O Lolium perenne
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Lolium perenne D0PQE8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains five potential N-glycosylation sites Lolium perenne

Source Tissue

Source Tissue Comment Organism Textmining
leaf in elongating leaf bases and mature leaf sheaths Lolium perenne
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1-kestotetraose + H2O low activity Lolium perenne ?
-
 ?
1-kestotriose + H2O low activity Lolium perenne ?
-
 ?
6,6-kestotetraose + H2O
-
 Lolium perenne ?
-
 ?
6-kestotriose + H2O best substrate Lolium perenne ?
-
 ?
6G-kestotriose + H2O
-
 Lolium perenne ?
-
 ?
fructan + H2O
-
 Lolium perenne ?
-
 ?
levan + H2O from Erwinia herbicola Lolium perenne ?
-
 ?
additional information purification of and assay with fructans from Lolium perenne, overview Lolium perenne ?
-
 ?

Synonyms

Synonyms Comment Organism
6-FEH
-
 Lolium perenne
6-FEHa
-
 Lolium perenne
6-fructan exohydrolase
-
 Lolium perenne
Lp6-FEHa
-
 Lolium perenne

General Information

General Information Comment Organism
evolution the enzyme's the catalytic triad is conserved among glycoside hydrolase family 32, GH32, members Lolium perenne
metabolism 6-FEHs are key enzymes associated with perennity of forage species Lolium perenne
physiological function Lolium perenne is a major forage grass species that accumulates fructans, mainly composed of beta(2,6)-linked fructose units. Fructans are mobilized through strongly increased activities of fructan exohydrolases (FEHs), sustaining regrowth following defoliation. Differences in the regulation of FEH activity among forage grasses influence their tolerance to defoliation Lolium perenne