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Literature summary for 3.2.1.154 extracted from
- Unexpected presence of graminan- and levan-type fructans in the evergreen frost-hardy eudicot Pachysandra terminalis (Buxaceae): purification, cloning, and functional analysis of a 6-SST/6-SFT enzyme (2011), Plant Physiol., 155, 603-614.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Pachysandra terminalis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22000 | - |
1 * 53000 + 1 * 22000, SDS-PAGE | Pachysandra terminalis |
| 53000 | - |
1 * 53000 + 1 * 22000, SDS-PAGE | Pachysandra terminalis |
| 70000 | - |
gel filtration | Pachysandra terminalis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pachysandra terminalis | E3PQS3 | Japanese spurge | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Pachysandra terminalis |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, concanavalin A column chromatography, Mono S (pH 4.5) column chromatography and Mono S (pH 4.0) column chromatography | Pachysandra terminalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + H2O | in vitro incubations with sucrose as a single substrate show a prominent formation of 6-kestotriose compared to 1-kestotriose and 6G-kestotriose (neokestose) and the formation of 1- and 6-kestotetraose from sucrose and 1-kestotriose | Pachysandra terminalis | D-fructose + D-glucose | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | 1 * 53000 + 1 * 22000, SDS-PAGE | Pachysandra terminalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-SST | - |
Pachysandra terminalis |
| fructan 6-exohydrolase | - |
Pachysandra terminalis |
| suc:fructan 6-fructosyltransferase | - |
Pachysandra terminalis |
| sucrose:fructan 6-fructosyltransferase | - |
Pachysandra terminalis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
about 20°C | Pachysandra terminalis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 15 | 45 | - |
Pachysandra terminalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | - |
Pachysandra terminalis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6.5 | - |
Pachysandra terminalis |
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