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Literature summary for 3.2.1.154 extracted from
- Characterization of an exo-beta-D-fructosidase from Streptococcus mutants ingbritt (1992), Microbiol. Immunol., 36, 643-647.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | 1 mM, 90% inhibition | Streptococcus mutans |  |
| Hg2+ | 1 mM, complete inhibition | Streptococcus mutans | |
| Zn2+ | 1 mM, 70% inhibition | Streptococcus mutans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Streptococcus mutans | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 127000 | - |
x * 127000, SDS-PAGE | Streptococcus mutans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus mutans | - |
ingbritt | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture supernatant | - |
Streptococcus mutans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.9 | - |
- |
Streptococcus mutans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| inulin + H2O | 13% of the activity with levan | Streptococcus mutans | fructose + ? | - |
? | |
| levan + H2O | exo-type mechanism | Streptococcus mutans | fructose + ? | - |
? | |
| raffinose + H2O | 5% of the activity with levan | Streptococcus mutans | ? | - |
? | |
| sucrose + H2O | 9% of the activity with levan | Streptococcus mutans | D-fructose + D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 127000, SDS-PAGE | Streptococcus mutans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
hydrolysis of levan | Streptococcus mutans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 60 | 55°C: maximal activity, 60°C: 61% of maximal activity | Streptococcus mutans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
hydrolysis of sucrose | Streptococcus mutans |
| 5.5 | - |
hydrolysis of levan | Streptococcus mutans |
| 6 | - |
hydrolysis of inulin | Streptococcus mutans |
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Release 2023.1 (January 2023)
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