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Literature summary for 3.2.1.154 extracted from

  • Burne, R.A.; Schilling, K.; Bowen, W.H.; Yasbin, R.E.
    Expression, purification, and characterization of an exo-beta-D-fructosidase of Streptococcus mutans (1987), J. Bacteriol., 169, 4507-4517.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.154

Cloned(Commentary)

Cloned (Comment) Organism
fruA is expressed under its own control in Escherichia coli JM83(pFRU1) Streptococcus mutans

Inhibitors

Inhibitors Comment Organism Structure
Ag2+ 1 mM, almost complete inhibition Streptococcus mutans
Cu2+ 1 mM, about 20% inhibition Streptococcus mutans
Hg2+ 0.01 mM, almost complete inhibition Streptococcus mutans
Mg2+ 1 mM, about 20% inhibition Streptococcus mutans
additional information no inhibition by F- or Tris. No evidence of product inhibition by fructose Streptococcus mutans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Streptococcus mutans 5737
-
cytoplasmic membrane
-
 Streptococcus mutans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ slight stimulation Streptococcus mutans
Mn2+ slight stimulation Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
140000
-
 x * 140000, SDS-PAGE Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans
-
 GS-5
-
Streptococcus mutans GS-5
-
 GS-5
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus mutans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
66.4
-
 enzyme from Streptococcus mutans Streptococcus mutans
204.5
-
 recombinant enzyme expressed in Escherichia coli Streptococcus mutans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O 34% of the activity with levan Streptococcus mutans fructose + ?
-
 ?
levan + H2O
-
 Streptococcus mutans fructose + ?
-
 ?
raffinose + H2O 12% of the activity with levan Streptococcus mutans ?
-
 ?
sucrose + H2O 21% of the activity with levan Streptococcus mutans D-fructose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
? x * 140000, SDS-PAGE Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
 sucrose degradation Streptococcus mutans
5.5
-
 reaction with levan or inulin Streptococcus mutans

pH Range

pH Minimum pH Maximum Comment Organism
4 6 pH 4.0: about 85% of maximal activity, pH 6.0: about 70% of maximal activity, reaction with sucrose Streptococcus mutans
4 7 pH 4.0: about 40% of maximal activity, pH 7.0: about 50% of maximal activity, reaction with levan or inulin Streptococcus mutans