Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.153 extracted from

  • Wei, H.; Zhao, H.; Su, T.; Bausewein, A.; Greiner, S.; Harms, K.; Rausch, T.
    Chicory R2R3-MYB transcription factors CiMYB5 and CiMYB3 regulate fructan 1-exohydrolase expression in response to abiotic stress and hormonal cues (2017), J. Exp. Bot., 68, 4323-4338 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.153

Cloned(Commentary)

Cloned (Comment) Organism
gene 1-FEH1, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview Cichorium intybus
gene 1-FEH2a, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview Cichorium intybus
gene 1-FEH2b, cloning from var. Zoom, cloning of transcription factors and target gene promoters using Phusion DNA polymerase, yeast-one-hybrid analysis, transcript levels for CiMYB1, CiMYB3, CiMYB4, and CiMYBa are strongly upregulated, being coinduced with 1-FEH1 and 1-FEH2, overview Cichorium intybus

Organism

Organism UniProt Comment Textmining
Cichorium intybus Q93X59 var. Zoom
-
Cichorium intybus Q93X60 var. Zoom
-
Cichorium intybus Q9FNS9 var. Zoom
-

Source Tissue

Source Tissue Comment Organism Textmining
hairy root
-
 Cichorium intybus
-
root
-
 Cichorium intybus
-
seedling
-
 Cichorium intybus
-
shoot
-
 Cichorium intybus
-
taproot
-
 Cichorium intybus
-

Synonyms

Synonyms Comment Organism
1-FEH2a
-
 Cichorium intybus
1-FEH2b
-
 Cichorium intybus
fructan 1-exohydrolase
-
 Cichorium intybus

Expression

Organism Comment Expression
Cichorium intybus expression of 1-FEH1 is reduced by zeatin down
Cichorium intybus expression of 1-FEH2a is reduced by zeatin down
Cichorium intybus expression of 1-FEH2b is reduced by zeatin down
Cichorium intybus several R2R3-MYB transcriptional regulators are analysed for their possible involvement in 1-FEH regulation via transient transactivation of 1-FEH target promoters and for in vivo co-expression with target genes under different stress and hormone treatments, overview additional information
Cichorium intybus CiMYB3 and CiMYB5 selectively enhance promoter activities of 1-FEH1, 1-FEH2a, and 1-FEH2b genes, without affecting promoter activities of fructosyltransferase genes. Both factors recognize the MYB-core motifs (C/TNGTTA/G) that are abundantly present in 1-FEH promoters. In chicory hairy root cultures CiMYB5 and CiMYB3 act as positive regulators of the fructan degradation pathway. Expression of 1-FEH1 is potently induced by abscisic acid, iodoacetic acid, and jasmonic acid. Cold exposure caused a substantial upregulation of transcript levels of 1-FEH1 across different plant organs up
Cichorium intybus CiMYB3 and CiMYB5 selectively enhance promoter activities of 1-FEH1, 1-FEH2a, and 1-FEH2b genes, without affecting promoter activities of fructosyltransferase genes. Both factors recognize the MYB-core motifs (C/TNGTTA/G) that are abundantly present in 1-FEH promoters. In chicory hairy root cultures CiMYB5 and CiMYB3 act as positive regulators of the fructan degradation pathway. Expression of 1-FEH2a is slightly induced by abscisic acid and iodoacetic acid, and more potently induced by jasmonic acid. Cold exposure causes a substantial upregulation of transcript levels of 1-FEH1 across different plant organs up
Cichorium intybus CiMYB3 and CiMYB5 selectively enhance promoter activities of 1-FEH1, 1-FEH2a, and 1-FEH2b genes, without affecting promoter activities of fructosyltransferase genes. Both factors recognize the MYB-core motifs (C/TNGTTA/G) that are abundantly present in 1-FEH promoters. In chicory hairy root cultures CiMYB5 and CiMYB3 act as positive regulators of the fructan degradation pathway. Expression of 1-FEH2b is slightly induced by abscisic acid and iodoacetic acid, and more potently induced by jasmonic acid. Cold exposure causes a substantial upregulation of transcript levels of 1-FEH1 across different plant organs up

General Information

General Information Comment Organism
metabolism in chicory hairy root cultures, transcription factor CiMYB5 displays co-expression with its target genes in response to different abiotic stress and phytohormone treatments, whereas correlations with CiMYB3 expression are less consistent. Oligofructan levels indicate that the metabolic response, while depending on the balance of the relative expression levels of fructan exohydrolases and fructosyltransferases, can be also affected by differential subcellular localization of different FEH isoforms. In chicory hairy root cultures CiMYB5 and CiMYB3 act as positive regulators of the fructan degradation pathway Cichorium intybus
physiological function in hairy root cultures with induced fructan accumulation, co-expression of transcription factor CiMYB5 and FEH genes is correlated with changes in oligofructan profiles upon stress and hormone treatments Cichorium intybus