Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.153 extracted from

  • Zhan, W.; Jin, L.; Jiao, J.; Zhang, X.; Zhang, Y.; Zhao, H.; Liang, M.
    Expression and purification of plant fructan exohydrolases and their potential applications in fructose production (2018), Int. J. Biol. Macromol., 108, 9-17 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene FEH-I, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 Cichorium intybus
gene FEH-IIa, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 Cichorium intybus
gene FEH-IIb, FEH isozyme sequences comparisons, phylogenetic analysis, recombinant expression of His-tagged enzyme in Pichia pastoris strain X-33 Cichorium intybus

Protein Variants

Protein Variants Comment Organism
additional information immobilization of purified recombinant isozyme Ci1-FEH IIa Cichorium intybus

Organism

Organism UniProt Comment Textmining
Cichorium intybus Q93X59
-
-
Cichorium intybus Q93X60
-
-
Cichorium intybus Q9FNS9
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Pichia pastoris strain X-33 by nickel affinity chromatography and ultrafiltration Cichorium intybus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
Cichorium intybus beta-D-fructose + sucrose
-
?
1-kestose + H2O best substrate Cichorium intybus beta-D-fructose + sucrose
-
?
fructofuranosylnystose + H2O
-
Cichorium intybus ?
-
?
fructofuranosylnystose + H2O best substrate Cichorium intybus ?
-
?
fructofuranosylnystose + H2O high activity Cichorium intybus ?
-
?
inulin + H2O best substrate Cichorium intybus ?
-
?
inulin + H2O high activity Cichorium intybus ?
-
?
additional information hydrolysis of Jerusalem artichoke tuber by immobilized isozyme Ci1-FEH IIa and analysis of carbohydrate profile. The fructan in the tuber is rapidly degraded to fructose by immobilized recombinant Ci1-FEH IIa. Substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH IIa has much higher activity than all other investigated FEH isozymes from Cichorium intybus and Helianthus tuberosus Cichorium intybus ?
-
?
additional information substrate specificity, overview. No activity with sucrose and levan Cichorium intybus ?
-
?
additional information substrate specificity, overview. No activity with sucrose and levan. Isozyme Ci1-FEH I shows quite low activity compared to other FEH isozymes analyzed from Cichorium intybus and Helianthus Cichorium intybus ?
-
?
nystose + H2O
-
Cichorium intybus ?
-
?
nystose + H2O best substrate Cichorium intybus ?
-
?
nystose + H2O high activity Cichorium intybus ?
-
?

Synonyms

Synonyms Comment Organism
Ci1-FEH I
-
Cichorium intybus
Ci1-FEH IIa
-
Cichorium intybus
Ci1-FEH IIb
-
Cichorium intybus
fructan 1-exohydrolase I
-
Cichorium intybus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Cichorium intybus
40 50
-
Cichorium intybus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 over 60% of maximal activity within this range Cichorium intybus
20 70 over 60% of maximal activity within this range Cichorium intybus
25 45 over 50% of maximal activity within this range Cichorium intybus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
Cichorium intybus
6
-
-
Cichorium intybus

pH Range

pH Minimum pH Maximum Comment Organism
4 6 over 80% of maximal activity within this range Cichorium intybus
4 6.5 over 50% of maximal activity within this range Cichorium intybus
4 7 over 40% of maximal activity within this range Cichorium intybus

General Information

General Information Comment Organism
evolution phylogenetic analysis of deduced amino acid sequences of plant fructan metabolism genes Cichorium intybus