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Literature summary for 3.2.1.153 extracted from

  • Le Roy, K.; Lammens, W.; Van Laere, A.; Van den Ende, W.
    Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolase (2008), New Phytol., 178, 572-580.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
heterologous expression in Pichia pastoris, pPICZalphaA vector Cichorium intybus

Protein Variants

Protein Variants Comment Organism
S101L neighbouring amino acid exchange influences the orientation of Trp82, thus switches the function of sucrose as inhibitor towards hydrolysation substrate, but only when present in high concentrations because substrate binding is inefficient Cichorium intybus

Inhibitors

Inhibitors Comment Organism Structure
sucrose inhibitor for wild-type 1-FEH IIa, weak hydrolysation product in S101L mutant Cichorium intybus

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Cichorium intybus hydrolytic cleavage of terminal fructosyl residues off inulin ?
-
?

Organism

Organism UniProt Comment Textmining
Cichorium intybus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Cichorium intybus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-kestose + H2O
-
Cichorium intybus ?
-
?
inulin + H2O 5% inulin from chicory, 30 degrees Celsius Cichorium intybus fructose + fructo-oligosaccharide + fructo-polysaccharide
-
?
additional information hydrolytic cleavage of terminal fructosyl residues off inulin Cichorium intybus ?
-
?
sucrose + H2O
-
Cichorium intybus D-fructose + D-glucose
-
?

Synonyms

Synonyms Comment Organism
1-FEH I
-
Cichorium intybus
1-FEH IIa
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Cichorium intybus
fructan 1-exohydrolase
-
Cichorium intybus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
sucrose 5 mM substrate: about zero activity in both enzyme variants, only at higher substrate concentrations (500-1000 mM) strongly raised activity: 10 mol fructose/mol enzyme Cichorium intybus
additional information
-
inulin 5 mM substrate: wild-type 1-FEH IIa 10 mol fructose/mol enzyme, S101L mutant about 2 mol fructose/mol enzyme Cichorium intybus
additional information
-
1-kestose 5 mM substrate: wild-type enzyme about 23 mol fructose/mol enzyme, mutant about 8 mol fructose/mol enzyme Cichorium intybus