Cloned (Comment) | Organism |
---|---|
heterologous expression in Pichia pastoris, pPICZalphaA vector | Cichorium intybus |
Protein Variants | Comment | Organism |
---|---|---|
S101L | neighbouring amino acid exchange influences the orientation of Trp82, thus switches the function of sucrose as inhibitor towards hydrolysation substrate, but only when present in high concentrations because substrate binding is inefficient | Cichorium intybus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sucrose | inhibitor for wild-type 1-FEH IIa, weak hydrolysation product in S101L mutant | Cichorium intybus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Cichorium intybus | hydrolytic cleavage of terminal fructosyl residues off inulin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cichorium intybus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cichorium intybus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-kestose + H2O | - |
Cichorium intybus | ? | - |
? | |
inulin + H2O | 5% inulin from chicory, 30 degrees Celsius | Cichorium intybus | fructose + fructo-oligosaccharide + fructo-polysaccharide | - |
? | |
additional information | hydrolytic cleavage of terminal fructosyl residues off inulin | Cichorium intybus | ? | - |
? | |
sucrose + H2O | - |
Cichorium intybus | D-fructose + D-glucose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-FEH I | - |
Cichorium intybus |
1-FEH IIa | - |
Cichorium intybus |
fructan 1-exohydrolase | - |
Cichorium intybus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
sucrose | 5 mM substrate: about zero activity in both enzyme variants, only at higher substrate concentrations (500-1000 mM) strongly raised activity: 10 mol fructose/mol enzyme | Cichorium intybus | |
additional information | - |
inulin | 5 mM substrate: wild-type 1-FEH IIa 10 mol fructose/mol enzyme, S101L mutant about 2 mol fructose/mol enzyme | Cichorium intybus | |
additional information | - |
1-kestose | 5 mM substrate: wild-type enzyme about 23 mol fructose/mol enzyme, mutant about 8 mol fructose/mol enzyme | Cichorium intybus |