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Literature summary for 3.2.1.153 extracted from

  • Van Den Ende, W.; Clerens, S.; Vergauwen, R.; Van Riet, L.; Van Laere, A.; Yoshida, M.; Kawakami, A.
    Fructan 1-exohydrolases. beta-(2,1)-trimmers during graminan biosynthesis in stems of wheat? Purification, characterization, mass mapping, and cloning of two fructan 1-exohydrolase isoforms (2003), Plant Physiol., 131, 621-631.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
isoenzyme 1-FEH w1 Triticum aestivum
isoenzyme 1-FEH w2 Triticum aestivum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7
-
 1-kestose isoenzyme1-FeH w1 Triticum aestivum
7
-
 1-kestose isoenzyme1-FeH w2 Triticum aestivum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, isoenzyme 1-FEH w1, SDS-PAGE Triticum aestivum
70000
-
 x * 70000, isoenzyme 1-FEH w2, SDS-PAGE Triticum aestivum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Triticum aestivum isoenzyme 1-FEH w2 might play a role as a beta-(2,1)-trimmer throughout the period of active graminan biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Triticum aestivum
-
 cv. Pajero
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycosylation on at least two of four potential N-glycosylation sites, isoenzyme 1-FEH w1 Triticum aestivum
glycoprotein glycosylation on at least two of four potential N-glycosylation sites, isoenzyme 1-FEH w2 Triticum aestivum

Purification (Commentary)

Purification (Comment) Organism
isoenzyme 1-FEH w1 Triticum aestivum
isoenzyme 1-FEH w2 Triticum aestivum

Source Tissue

Source Tissue Comment Organism Textmining
stem
-
 Triticum aestivum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12.2
-
 isoenzyme 1-FEH w2 Triticum aestivum
14.6
-
 isoenzyme 1-FEH w1 Triticum aestivum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1-nystose + H2O 72% of the activity with 1-kestose, isoenzyme 1-FEH w1 Triticum aestivum ?
-
 ?
1,1-nystose + H2O 83% of the activity with 1-kestose, isoenzyme 1-FEH w2 Triticum aestivum ?
-
 ?
1-kestose + H2O
-
 Triticum aestivum ?
-
 ?
6-kestose + H2O 0.7% of the activity with 1-kestose, isoenzyme 1-FEH w1 Triticum aestivum ?
-
 ?
6-kestose + H2O 1% of the activity with 1-kestose, isoenzyme 1-FEH w2 Triticum aestivum ?
-
 ?
inulin + H2O 22% of the activity with 1-kestose, isoenzyme 1-FEH w2 Triticum aestivum ?
-
 ?
inulin + H2O 39% of the activity with 1-kestose, isoenzyme 1-FEH w1 Triticum aestivum ?
-
 ?
levan + H2O 3% of the activity with 1-kestose, isoenzyme 1-FEH w2 Triticum aestivum ?
-
 ?
levan + H2O 4% of the activity with 1-kestose, isoenzyme 1-FEH w1 Triticum aestivum ?
-
 ?
additional information isoenzyme 1-FEH w2 might play a role as a beta-(2,1)-trimmer throughout the period of active graminan biosynthesis Triticum aestivum ?
-
 ?
neokestose + H2O 3% of the activity with 1-kestose, isoenzyme 1-FEH w2 Triticum aestivum ?
-
 ?
neokestose + H2O 4% of the activity with 1-kestose, isoenzyme 1-FEH w1 Triticum aestivum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, isoenzyme 1-FEH w1, SDS-PAGE Triticum aestivum
? x * 70000, isoenzyme 1-FEH w2, SDS-PAGE Triticum aestivum

Synonyms

Synonyms Comment Organism
1-FEH w1
-
 Triticum aestivum
1-FEH w2
-
 Triticum aestivum
fructan 1-exohydrolase w1
-
 Triticum aestivum
fructan 1-exohydrolase w2
-
 Triticum aestivum

pI Value

Organism Comment pI Value Maximum pI Value
Triticum aestivum calculation from cDNA, isoenzyme 1-FEH w2
-
 4.78
Triticum aestivum calculation from cDNA, isoenzyme 1-FEH w1
-
 4.79