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Literature summary for 3.2.1.152 extracted from
- Substrate specificity and molecular cloning of the lily endo-beta-mannosidase acting on N-glycan (2005), J. Biochem., 137, 87-93.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Lilium longiflorum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.2 | - |
Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine | pH 5.0, 37°C | Lilium longiflorum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
alphabetagamma, 1 * 42000 + 1 * 31000 + 1 * 28000, SDS-PAGE | Lilium longiflorum |
| 31000 | - |
alphabetagamma, 1 * 42000 + 1 * 31000 + 1 * 28000, SDS-PAGE | Lilium longiflorum |
| 42000 | - |
alphabetagamma, 1 * 42000 + 1 * 31000 + 1 * 28000, SDS-PAGE | Lilium longiflorum |
| 108000 | - |
calculated from the deduced amino acid sequence | Lilium longiflorum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Man-beta-(1-4)GlcNAc + H2O | Lilium longiflorum | hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Lilium longiflorum | Q5H7P5 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | enzyme is encoded by a single cDNA, translation product is posttranslationally processed by proteolysis | Lilium longiflorum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bulb | highest activity | Lilium longiflorum | - |
| flower | - |
Lilium longiflorum | - |
| leaf | - |
Lilium longiflorum | - |
| pistil | - |
Lilium longiflorum | - |
| stamen | - |
Lilium longiflorum | - |
| stem | - |
Lilium longiflorum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Man-alpha-(1-6)Man-beta-(1-4)GlcNAc-beta-(1-4)GlcNAc-pyridylamine + H2O | - |
Lilium longiflorum | GlcNAc-beta-(1-4)GlcNAc-pyridylamine + Man-alpha-(1-6)Man | - |
? | |
| Man-beta-(1-4)GlcNAc + H2O | hydrolysis of Man-beta-(1-4)GlcNAc linkage in the trimannosyl core structure of N-glycans | Lilium longiflorum | ? | - |
? | |
| additional information | enzyme does not hydrolyze pyridylamino-sugar chains containing Man-alpha-(1-3)Man-beta or Xylose-beta-(1-2)Man-beta, enzyme does not hydrolyze p-nitrophenyl-beta-mannoside | Lilium longiflorum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | alphabetagamma, 1 * 42000 + 1 * 31000 + 1 * 28000, SDS-PAGE | Lilium longiflorum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-beta-mannosidase | - |
Lilium longiflorum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Lilium longiflorum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 6 | - |
Lilium longiflorum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | unstable below pH 3 and above pH 8 | Lilium longiflorum |
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