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Literature summary for 3.2.1.151 extracted from
- Xyloglucan breakdown by endo-xyloglucanase family 74 from Aspergillus fumigatus (2017), Appl. Microbiol. Biotechnol., 101, 2893-2903 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AFUA_8G01490, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, detailed overview. Recombinant enzyme expression in Aspergillus nidulans strain A773 | Aspergillus fumigatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics with substrate XyGt, pH 5.5, 50°C, Km is 1.5 mg/ml | Aspergillus fumigatus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | Aspergillus fumigatus | - |
xyloglucan oligosaccharides | - |
? |  |
| xyloglucan + H2O | Aspergillus fumigatus CBS 101355 | - |
xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | Aspergillus fumigatus ATCC MYA-4609 | - |
xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | Aspergillus fumigatus FGSC A1100 | - |
xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | Aspergillus fumigatus Af293 | - |
xyloglucan oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | Q4WB93 | i.e. Aspergillus fumigatus | - |
| Aspergillus fumigatus Af293 | Q4WB93 | i.e. Aspergillus fumigatus | - |
| Aspergillus fumigatus ATCC MYA-4609 | Q4WB93 | i.e. Aspergillus fumigatus | - |
| Aspergillus fumigatus CBS 101355 | Q4WB93 | i.e. Aspergillus fumigatus | - |
| Aspergillus fumigatus FGSC A1100 | Q4WB93 | i.e. Aspergillus fumigatus | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the enzyme has five predicted N-glycosylation sites | Aspergillus fumigatus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus fumigatus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.9 | - |
purified recombinant enzyme, pH 5.5, 50°C, substrate XyGt | Aspergillus fumigatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | - |
Aspergillus fumigatus | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides | Aspergillus fumigatus | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | - |
Aspergillus fumigatus CBS 101355 | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides | Aspergillus fumigatus CBS 101355 | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | - |
Aspergillus fumigatus ATCC MYA-4609 | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides | Aspergillus fumigatus ATCC MYA-4609 | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | - |
Aspergillus fumigatus FGSC A1100 | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides | Aspergillus fumigatus FGSC A1100 | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | - |
Aspergillus fumigatus Af293 | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | xyloglucan from tamarind (i.e. XyGt, MW about 225 kDa, sugar composition: xylose 34%, glucose 45%, galactose 18%, and arabinose 3%) is used as substrate for activity assays. Oligosaccharides from xyloglucan hydrolysis by AfXEG74 are derivatized with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) by reductive amination and analyzed by capillary zone electrophoresis with a laser-induced fluorescence detector. Generation of XyGO with DP4 as the major product, assignment and interpretation of MALDI-TOF MS peaks after tamarind xyloglucan hydrolysis by AfXEG74, overview. Substrate specificity is evaluated employing many substrates, including mannan, microcrystalline cellulose (Avicel and Sigmacell 50), carboxymethyl cellulose (CMC), larch arabinogalactan, chitosan, xylan from beechwood, lichenan, rye arabinoxylan, barley beta-glucan, and XyGt. AfXEG74 does not cleave short xyloglucan oligosaccharides | Aspergillus fumigatus Af293 | xyloglucan oligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 85000, sequence calculation, x * 100000, recombinant enzyme, SDS-PAGE | Aspergillus fumigatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AfXEG74 | - |
Aspergillus fumigatus |
| AspGD | - |
Aspergillus fumigatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
recombinant enzyme, with substrate XyGt | Aspergillus fumigatus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 65 | activity range, recombinant enzyme, profile overview | Aspergillus fumigatus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 57.2 | - |
melting temperature (Tm) of AfXEG74 is 57.2°C | Aspergillus fumigatus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16.4 | - |
xyloglucan | recombinant enzyme, pH 5.5, 50°C | Aspergillus fumigatus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
recombinant enzyme, with substrate XyGt | Aspergillus fumigatus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.5 | activity range, recombinant enzyme, profile overview | Aspergillus fumigatus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Aspergillus fumigatus | sequence calculation | - |
5.11 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the endo-xyloglucanase family 74, GH74, family of enzymes. Phylogenetic diversity and catalytic mechanism of GH74 xyloglucanases from Ascomycete fungi, analysis of genome-wide distribution of GH74-encoding genes in ascomycetes, overview | Aspergillus fumigatus |
| additional information | mode of action of enzyme AfXEG74, overview. AfXEG74 hydrolyzes xyloglucan from tamarind (XyGt) by acting mainly on unbranched glucosyl residues (G), either before or after this position. In some cases, XX positions can also be attacked at specific points, for example when this motif is linked to the XG position that occurs in regions deprived of galactosyl branches | Aspergillus fumigatus |
| physiological function | enzyme AfXEG74 has no CBM modul and shows high specificity in xyloglucan hydrolysis. AfXEG74 does not cleave short xyloglucan oligosaccharides | Aspergillus fumigatus |
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Release 2023.1 (January 2023)
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