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Literature summary for 3.2.1.151 extracted from

  • Shi, Y.; Zhu, X.; Miller, J.; Gregson, T.; Zheng, S.; Fry, S.
    Distinct catalytic capacities of two aluminium-repressed Arabidopsis thaliana xyloglucan endotransglucosylase/hydrolases, XTH15 and XTH31, heterologously produced in Pichia (2015), Phytochemistry, 112, 160-169 .
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Cloned(Commentary)

Cloned (Comment) Organism
gene XTH15, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted Arabidopsis thaliana
gene XTH31, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information the XEH activity of XTH31 gives a hyperbola, and the Km for tamarind xyloglucan is about 1.6 mg/ml Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xyloglucan + H2O Arabidopsis thaliana
-
 xyloglucooligosaccharides
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P93046
-
-
Arabidopsis thaliana Q38911
-
-
no activity in Pichia pastoris
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information enzyme XTH15 is strongly expressed in young, rapidly growing organs, but particularly in 24-h imbibed seeds, the seedling hypocotyl and root, and early-stage flowers (especially in the carpels) Arabidopsis thaliana
-
additional information enzyme XTH31 is strongly expressed in young, rapidly growing organs. XTH31 is more specialised, being expressed in fewer organs (24-h imbibed seeds, hypocotyl, root elongation zone, cotyledons and immature leaves), albeit at higher concentrations Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme XTH15, a classical group-I/II xyloglucan endotransglucosylase/hydrolase (XTH), has high xyloglucan endotransglucosylase (XET) and undetectable xyloglucan hydrolase (XEH) activity in vitro Arabidopsis thaliana ?
-
 ?
additional information hydrolysis products formed from [rt-3H]xyloglucan by XTH31 are analysed by GPC Arabidopsis thaliana ?
-
 ?
tamarind xyloglucan + H2O XTH31 cleaves the substrate from about 186 kDa to about 6 kDa (median sizes) within 40 h. The enzyme does not have a preferred cleavage site close to either terminus Arabidopsis thaliana tamarind xyloglucooligosaccharides
-
 ?
xyloglucan + H2O
-
 Arabidopsis thaliana xyloglucooligosaccharides
-
 ?

Synonyms

Synonyms Comment Organism
More see also EC 2.4.1.207 Arabidopsis thaliana
XEH
-
 Arabidopsis thaliana
XTH15
-
 Arabidopsis thaliana
XTH31
-
 Arabidopsis thaliana
xyloglucan endotransglucosylase/hydrolase
-
 Arabidopsis thaliana
xyloglucan hydrolase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Arabidopsis thaliana
5.5
-
 assay at Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6.2 25% of maximal activity at pH 3.5, maximal activity at pH 4.5, steady promotion of XTH31 XEH activity as the pH drops from pH 6.2 to pH 4.4 Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function a role for enzyme XTH31 in acid growth. Isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity Arabidopsis thaliana
physiological function isozymes XTH15 and XTH31 are both strongly expressed in young, rapidly growing organs, suggesting that they play roles in cell expansion. In vitro, XTH15 has very high transglucanase (endotransglucosylase, XET) but undetectable hydrolytic activity (glucanase, XEH). In contrast, XTH31 has very high XEH activity and only slight XET activity Arabidopsis thaliana