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Literature summary for 3.2.1.151 extracted from
- Identification and characterization of an acidic and acid-stable endoxyloglucanase from Penicillium oxalicum (2016), Int. J. Biol. Macromol., 86, 512-518 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no effect on enzyme activity by 1-5 mM EDTA | Penicillium oxalicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PoxXEG12A, sequence comparisons, recombinant expression of His-tagged enzyme in Pichia pastoris strain GS115 under control of methanol-inducible AOX1 promoter | Penicillium oxalicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight inhibition at 1-5 mM | Penicillium oxalicum |  |
| Co2+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| Cr2+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| Cu2+ | 65% inhibition at 5 mM | Penicillium oxalicum | |
| Fe2+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| Fe3+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| K+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| Li+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| Mg2+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| additional information | no effect on enzyme activity by 1-5 mM EDTA | Penicillium oxalicum | |
| Na+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| Ni2+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
| Zn2+ | slight inhibition at 1-5 mM | Penicillium oxalicum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium oxalicum | A0A0A7RRF2 | - |
- |
| Penicillium oxalicum HP7-1 | A0A0A7RRF2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Pichia pastoris strain GS115 by nickel affinity chromatography | Penicillium oxalicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 172 | - |
recombinant enzyme, substrate tamarind xyloglucan, pH 5.0, 60°C | Penicillium oxalicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates | Penicillium oxalicum | ? | - |
? | |
| additional information | substrate specificity, the enzyme is highly specific for tamarind xyloglucan, it shows no activity against lichenan, methylcellulose, carboxymethyl cellulose, Avicel, xylan from beechwood, xylan from birch wood, barley glucan, laminarin, and galactan as substrates | Penicillium oxalicum HP7-1 | ? | - |
? | |
| tamarind seed xyloglucan + H2O | the enzyme specifically hydrolyzes tamarind xyloglucan in endo-acting mode | Penicillium oxalicum | xyloglucooligosaccharides | - |
? | |
| tamarind seed xyloglucan + H2O | the enzyme specifically hydrolyzes tamarind xyloglucan in endo-acting mode | Penicillium oxalicum HP7-1 | xyloglucooligosaccharides | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoxyloglucanase | - |
Penicillium oxalicum |
| POXEG12A | gene name, UniProt | Penicillium oxalicum |
| PoxXEG12A | - |
Penicillium oxalicum |
| xyloglucanase | - |
Penicillium oxalicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 60 | - |
Penicillium oxalicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 70 | activity range, profile overview. The enzyme shows over 50% of maximal activity at 45-65°C | Penicillium oxalicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
recombinant enzyme, pH 5.0, completely stable up to | Penicillium oxalicum |
| 45 | 50 | recombinant enzyme, pH 5.0, 40% activity remaining, inactivation at 50°C | Penicillium oxalicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5.5 | - |
Penicillium oxalicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8.5 | recombinant enzyme, activity range, profile overview. The enzyme shows over 90% of maximal activity at pH 3.5-7.0 | Penicillium oxalicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7 | recombinant enzyme, 37°C, stable at | Penicillium oxalicum |
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Release 2023.1 (January 2023)
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