Cloned (Comment) | Organism |
---|---|
gene xeg1, quantitative expression analysis, recombinant expression in Saccharomyces cerevisiae strain INVSc1 using the galactose inducible GAL1 promoter, heterologous expression of gene xeg1 in yeast shows specificity towards xyloglucan | Acromyrmex echinatior |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acromyrmex echinatior | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | highly active enzyme Xeg1 in symbiotic ant fecal fluid. The enzyme is significantly more expressed in the gongylidia than in normal mycelium | Acromyrmex echinatior | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
barley beta-glucan + H2O | lower activity compared to xyloglucan | Acromyrmex echinatior | ? | - |
? | |
hydroxyethyl cellulose + H2O | very low activity | Acromyrmex echinatior | ? | - |
? | |
pachyman + H2O | lower activity compared to xyloglucan | Acromyrmex echinatior | ? | - |
? | |
xyloglucan + H2O | substrate specificity of fungal Xeg1 towards xyloglucan | Acromyrmex echinatior | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-xyloglucanase | - |
Acromyrmex echinatior |
Xeg1 | - |
Acromyrmex echinatior |
xyloglucanase | - |
Acromyrmex echinatior |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 12, GH12 | Acromyrmex echinatior |
physiological function | the fungal symbiont provides a single xyloglucanase (Xeg1) to its ant farmers by upregulating the expression of this protein in the inflated hyphal tips (gongylidia) that the ants ingest. Similar to other enzymes ingested this way, also Xeg1 is not digested but vectored to the fresh leaf-fragment pulp at the top of fungus gardens via ant fecal fluid. Xeg1 is 4-5 times more active in fecal fluid when ants ingest their normal fungal food, compared to a sucrose control diet, as expected when they cannot produce Xeg1 themselves. Ingestion of the enzyme by the ants for transfer to the fecal fluid is actively promoted by the fungal symbiont | Acromyrmex echinatior |