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Literature summary for 3.2.1.151 extracted from

  • Ariza, A.; Ekloef, J.M.; Spadiut, O.; Offen, W.A.; Roberts, S.M.; Besenmatter, W.; Friis, E.P.; Skjot, M.; Wilson, K.S.; Brumer, H.; Davies, G.
    Structure and activity of Paenibacillus polymyxa xyloglucanase from glycoside hydrolase family 44 (2011), J. Biol. Chem., 286, 33890-33900.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.151

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1 M bis-Tris, pH 6.5, 0.2 M Li2SO4, 25% (w/v) PEG 3350 Paenibacillus polymyxa

Protein Variants

Protein Variants Comment Organism
E358S nucleophile mutant Paenibacillus polymyxa
K129A/R156Y increased stability mutant Paenibacillus polymyxa

Inhibitors

Inhibitors Comment Organism Structure
beta-1,4-cellobiosyloxazine competitive inhibitor Paenibacillus polymyxa
beta-1,4-glucosyl-noeuromycin competitive inhibitor Paenibacillus polymyxa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.029
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
0.134
-
 Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
0.361
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
0.998
-
 Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa

Organism

Organism UniProt Comment Textmining
Paenibacillus polymyxa
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O 3.8% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta Paenibacillus polymyxa 2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
 ?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O 100% activity Paenibacillus polymyxa 2-chloro-4-nitrophenol + Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta
-
 ?
2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O 1.3% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta Paenibacillus polymyxa 2-chloro-4-nitrophenol + ?
-
 ?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
2-chloro-4-nitrophenyl Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O 19.4% activity compared to 2-chloro-4-nitrophenyl-Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta Paenibacillus polymyxa 2-chloro-4-nitrophenol + ?
-
 ?
barley beta-glucan + H2O 87% activity compared to tamarind xyloglucan Paenibacillus polymyxa ?
-
 ?
carboxymethylcellulose + H2O high activity Paenibacillus polymyxa ?
-
 ?
carob galactomannan + H2O 4% activity compared to tamarind xyloglucan Paenibacillus polymyxa ?
-
 ?
cellohexaose + H2O
-
 Paenibacillus polymyxa ?
-
 ?
cellopentaose + H2O
-
 Paenibacillus polymyxa ?
-
 ?
cellotetraose + H2O
-
 Paenibacillus polymyxa ?
-
 ?
Glcbeta(1->4)[Xylalpha(1->6)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)[Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
hydroxyethyl cellulose + H2O 65% activity compared to tamarind xyloglucan Paenibacillus polymyxa ?
-
 ?
additional information no activity with 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta, cellotriose, and cellobiose Paenibacillus polymyxa ?
-
 ?
additional information the enzyme demonstrates a broad substrate specificity for polysaccharides containing beta-1,4 linkages and displays an unusual specificity on defined xyloglucan oligosaccharides, cleaving the XXXG-XXXG repeat into XXX and GXXXG Paenibacillus polymyxa ?
-
 ?
tamarind xyloglucan + H2O highest activity (100%) Paenibacillus polymyxa ?
-
 ?
wheat arabinoxylan + H2O 15% activity compared to tamarind xyloglucan Paenibacillus polymyxa ?
-
 ?
Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta + H2O
-
 Paenibacillus polymyxa ?
-
 ?
xyloglucan + H2O
-
 Paenibacillus polymyxa xyloglucan oligosaccharides
-
 ?

Synonyms

Synonyms Comment Organism
endo-beta-1,4-(xylo)glucan hydrolase
-
 Paenibacillus polymyxa
XG44
-
 Paenibacillus polymyxa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.96
-
 Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
7.34
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
14.01
-
 Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
15.93
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 for hydrolysis of tamarind xyloglucan Paenibacillus polymyxa

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.98
-
 Xylalpha(1->6)Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)[Galbeta(1->2)Xylalpha(1->6)]Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
20.33
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
104.7
-
 Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Xylalpha(1->6)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa
541.7
-
 2-chloro-4-nitrophenyl Glcbeta(1->4)Glcbeta(1->4)Glcbeta(1->4)Glcbeta apparent value, at pH 6.0 and 30°C Paenibacillus polymyxa