Literature summary for 3.2.1.151 extracted from

Gullfot, F.; Ibatullin, F.; Sundqvist, G.; Davies, G.; Brumer, H.
Functional characterization of xyloglucan glycosynthases from GH7, GH12, and GH16 scaffolds (2009), Biomacromolecules, 10, 1782-1788.

Search for more literature for 3.2.1.151

Application

Application	Comment	Organism
synthesis	mutant engineered xyloglucanases acting as glycosynthases are emerging as useful tools for the synthesis of large, complex polysaccharides, method development for robust and versatile method for the preparative synthesis of homogeneous xyloglucans with regular substitution patterns not available in nature, overview	Bacillus licheniformis
synthesis	mutant engineered xyloglucanases acting as glycosynthases are emerging as useful tools for the synthesis of large, complex polysaccharides, method development for robust and versatile method for the preparative synthesis of homogeneous xyloglucans with regular substitution patterns not available in nature, overview	Tropaeolum majus

Protein Variants

Protein Variants	Comment	Organism
E155A	the mutant acts as glucosynthase and can perform the condensation of xyloglucosyl fluorides, albeit at poor rates	Bacillus licheniformis
E94A	the GH16 xyloglucan hydrolase mutant of TmNXG1 acting as glycosynthase is capable of synthesizing XLLG-based xyloglucan oligosaccharides at rates feasible for preparative synthesis, thus providing an essential expansion of product ranges	Tropaeolum majus
additional information	mutation and engineering of wild-type xyloglucanase to glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates	Bacillus licheniformis
additional information	mutation and engineering of wild-type xyloglucanase to glycosynthases, hydrolytically inactive mutant glycosidases that catalyze glycosylation reactions using glycosyl fluoride donor substrates	Tropaeolum majus

Organism

Organism	UniProt	Comment	Textmining
Bacillus licheniformis	-	-	-
Tropaeolum majus	-	-	-

Synonyms

Synonyms	Comment	Organism
BlXG12	-	Bacillus licheniformis
GH12 xyloglucanase	-	Bacillus licheniformis
GH16 xyloglucan hydrolase	-	Tropaeolum majus
TmNXG1	-	Tropaeolum majus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at, mutant enzyme	Bacillus licheniformis
30	-	assay at, mutant enzyme	Tropaeolum majus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	assay at, mutant enzyme	Tropaeolum majus
6	-	assay at, mutant enzyme	Bacillus licheniformis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)