Cloned (Comment) | Organism |
---|---|
expression in Aspergillus niger | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km value for xyloglucan is 0.39 mg/ml at 50°C, pH 5.0 | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus niger | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23300 | - |
x * 23300, calculated, mature protein, x * 32000, SDS-PAGE | Aspergillus niger |
32000 | - |
x * 23300, calculated, mature protein, x * 32000, SDS-PAGE | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | A1XP58 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
no glycoprotein | N-glycosylation site at N32 is not glycosylated | Aspergillus niger |
proteolytic modification | sequence contains a signal peptide of 15 amino acids | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture fluid | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
83 | - |
50°C, pH 5.0 | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carboxymethyl curdlan + H2O | - |
Aspergillus niger | carboxymethyl curdlan oligosaccharides | less than 2% of the activity with xyloglucan | ? | |
carboxymethyl pachyman + H2O | - |
Aspergillus niger | carboxymethyl pachyman oligosaccharides | less than 2% of the activity with xyloglucan | ? | |
additional information | no substrate: carboxymethyl cellulose-4M, xylan from birch, beech or oat spelt, lichenan, laminarin, pullulan, galactomannan, glucomannan | Aspergillus niger | ? | - |
? | |
xyloglucan + H2O | substrate xyloglucan from tamarind seed, cleavage at unbranched glucose in the backbone | Aspergillus niger | xyloglucan oligosaccharides | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 23300, calculated, mature protein, x * 32000, SDS-PAGE | Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 60 | - |
Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
34 | - |
xyloglucan | 50°C, pH 5.0 | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus niger | calculated | - |
3.6 |