Literature summary for 3.2.1.151 extracted from

Baumann, M.J.; Ekloef, J.M.; Michel, G.; Kallas, A.M.; Teeri, T.T.; Czjzek, M.; Brumer, H.
Structural evidence for the evolution of xyloglucanase activity from xyloglucan endo-transglycosylases: Biological implications for cell wall metabolism (2007), Plant Cell, 19, 1947-1963.

Search for more literature for 3.2.1.151

Crystallization (Commentary)

Crystallization (Comment)	Organism
isoforms NXG1 and NXG2, comparison with data from the strict endo-transglycolase from Populus tremula x Populus tremuloides	Tropaeolum majus

Protein Variants

Protein Variants	Comment	Organism
DELTAYNIIG	loop deletion variant of isoform NXG1, structurally similar to the strict endo-transglycolase from Populus tremula x Populus tremuloides. Mutant has a greatly increased transglycosylation:hydrolysis ratio	Tropaeolum majus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.08	-	xyloglucan	pH 4.8, isoform NXG1	Tropaeolum majus
0.5	-	xyloglucan	pH 4.8, isoform NXG1, transglycosylation of xyloglucan	Tropaeolum majus
2	-	xyloglucan	pH 4.8, mutant DELTAYNIIG of isoform NXG1, transglycosylation of xyloglucan	Tropaeolum majus

Organism

Organism	UniProt	Comment	Textmining
Tropaeolum majus	-	isoforms NXG1 and NXG2	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
xyloglucan + H2O	-	Tropaeolum majus	xyloglucan oligosaccharides	-	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.012	-	xyloglucan	pH 4.8, mutant DELTAYNIIG of isoform NXG1, hydrolysis of xyloglucan	Tropaeolum majus
0.013	-	xyloglucan	pH 4.8, isoform NXG1, transglycosylation of xyloglucan	Tropaeolum majus
0.015	-	xyloglucan	pH 4.8, mutant DELTAYNIIG of isoform NXG1, transglycosylation of xyloglucan	Tropaeolum majus
0.08	-	xyloglucan	pH 4.8, isoform NXG1, hydrolysis of xyloglucan	Tropaeolum majus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.8	-	both isoforms NXG1 and NXG2	Tropaeolum majus

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)