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Literature summary for 3.2.1.151 extracted from

  • Yaoi, K.; Mitsuishi, Y.
    Purification, characterization, cDNA cloning, and expression of a xyloglucan endoglucanase from Geotrichum sp. M128 (2004), FEBS Lett., 560, 45-50.
    View publication on PubMed
Search for more literature for 3.2.1.151

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geotrichum sp. M128

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
 x * 80000, SDS-PAGE Geotrichum sp. M128
80500
-
 x * 80500, MALDI-TOF Geotrichum sp. M128

Organism

Organism UniProt Comment Textmining
Geotrichum sp. M128
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geotrichum sp. M128

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
68
-
-
 Geotrichum sp. M128

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GGXXXG + H2O
-
 Geotrichum sp. M128 Glc(beta1-4)Glc + XXXG
-
 ?
GXGXXXG + H2O
-
 Geotrichum sp. M128 GXG + XXXG
-
 ?
additional information no activity with carboxymethylcellulose, Avicel and barley 1,3-1,4-beta-glucan. NO activity with GXXXG and XXXGG. Low activity with GGGX and XXXGX Geotrichum sp. M128 ?
-
 ?
XGXXXG + H2O
-
 Geotrichum sp. M128 XG + XXXG
-
 ?
XXGXXXG + H2O
-
 Geotrichum sp. M128 XXG + XXXG
-
 ?
XXXGXX + H2O
-
 Geotrichum sp. M128 XXXG + XX
-
 ?
XXXGXXX + H2O
-
 Geotrichum sp. M128 XXXG + XX
-
 ?
XXXXGXXXXG + H2O
-
 Geotrichum sp. M128 XXXXG
-
 ?
xyloglucan + H2O tamarind and pea xyloglucan. The enzyme has at least four subsites (-2 to -2) and specifically recognizes xylose branching at the +1 and +2 sites, branching at the -1 site decreases the activity Geotrichum sp. M128 xyloglucan oligosaccharides
-
 ?

Subunits

Subunits Comment Organism
? x * 80000, SDS-PAGE Geotrichum sp. M128
? x * 80500, MALDI-TOF Geotrichum sp. M128

Synonyms

Synonyms Comment Organism
xyloglucan-specific endo-beta-1,4-glucanase
-
 Geotrichum sp. M128

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Geotrichum sp. M128

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 5.5, 10 min, more than 90% of its activity is retained Geotrichum sp. M128

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Geotrichum sp. M128

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 25°C, stable Geotrichum sp. M128
5.5 6.5 45°C, stable Geotrichum sp. M128

pI Value

Organism Comment pI Value Maximum pI Value
Geotrichum sp. M128 chromatofocusing
-
 4.8