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Literature summary for 3.2.1.151 extracted from
- A xyloglucan-specific endo-1,4-beta-glucanase isolated from auxin-treated pea stems (1997), Plant Physiol., 114, 661-667.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.64 | - |
xyloglucan | pH 6.2, 35°C | Pisum sativum |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 70000 | - |
gel filtration | Pisum sativum |
| 77000 | - |
SDS-PAGE | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xyloglucan + H2O | Pisum sativum | - |
xyloglucan oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
auxin-treated pea, var. Alaska | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| homogeneity | Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| stem | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.294 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no substrate: swollen cellulose | Pisum sativum | ? | - |
? | |
| additional information | no substrate: (1--3,1--4)-beta-glucan | Pisum sativum | ? | - |
? | |
| additional information | no substrate: carboxymethylcellulose | Pisum sativum | ? | - |
? | |
| xyloglucan + H2O | - |
Pisum sativum | xyloglucan oligosaccharides | - |
? | |
| xyloglucan + H2O | specifically cleaves the 1,4-beta-glucosyl linkages of the xyloglucan backbone to yield mainly nona- and heptasaccharides | Pisum sativum | xyloglucan oligosaccharides | mainly nona- and heptasaccharides | ? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Pisum sativum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | - |
- |
Pisum sativum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | active between pH 4.5 and pH 8 | Pisum sativum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Pisum sativum | pH gradient 6-9 | - |
8.1 |
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Release 2023.1 (January 2023)
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