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Literature summary for 3.2.1.15 extracted from

  • Fratebianchi, D.; Cavello, I.A.; Cavalitto, S.F.
    Purification and biochemical and kinetic properties of an endo-polygalacturonase from the industrial fungus Aspergillus sojae (2017), J. Mol. Microbiol. Biotechnol., 27, 102-109 .
    View publication on PubMed
Search for more literature for 3.2.1.15

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition at 1 mM Aspergillus sojae
additional information not affected by 2-mercaptoethanol, 15% (v/v) ethanol or 100 mg/l SO2 Aspergillus sojae
trigalacturonate
-
 Aspergillus sojae

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Ca2+, K+, Mg2+, and Zn2+ Aspergillus sojae

Organism

Organism UniProt Comment Textmining
Aspergillus sojae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, SP Sepharose column chromatography, and Superdex 75 gel filtration Aspergillus sojae

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin + H2O
-
 Aspergillus sojae ?
-
 ?
lemon protopectin + H2O
-
 Aspergillus sojae ?
-
 ?
polygalacturonate + H2O
-
 Aspergillus sojae trigalacturonate + digalactuornate + D-galacturonic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 47000, SDS-PAGE Aspergillus sojae

Synonyms

Synonyms Comment Organism
endo-polygalacturonase
-
 Aspergillus sojae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 the enzyme presents a half-life of 2 h at 50°C. The enzyme is fully thermostable up to 45°C, whereas 25% of the initial activity is retained after 210 min of incubation at 50°C Aspergillus sojae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 with citrus pectin as substrate Aspergillus sojae
4.3
-
 with lemon protopectin as substrate Aspergillus sojae
5
-
 with polygalacturonate as substrate Aspergillus sojae

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 6.5 after 2 h of incubation, the purified enzyme retains more than 75% of its initial activity between the pH values of 2.0 and 6.5, and shows up to 55% residual activity within a very low pH interval (1.0-2.0). After 24 h in the pH range of 1.0-3.0 the enzyme retains 35% of its initial activity at pH 1.0 and more than 75% from pH 1.5 onwards Aspergillus sojae

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.6
-
 trigalacturonate at pH 5.0 and 20°C Aspergillus sojae

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus sojae isoelectric focusing
-
 4.2