Cloned (Comment) | Organism |
---|---|
expressed in Saccharomyces cerevisiae strain EBY100 | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 92% residual activity at 1 mM | Aspergillus niger | |
Co2+ | about 75% residual activity at 1 mM | Aspergillus niger | |
Mn2+ | about 55% residual activity at 1 mM | Aspergillus niger | |
Ni2+ | about 82% residual activity at 1 mM | Aspergillus niger | |
Pb2+ | about 38% residual activity at 1 mM | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 125% activity at 1 mM | Aspergillus niger | |
Cu2+ | about 123% activity at 1 mM | Aspergillus niger | |
Fe2+ | about 115% activity at 1 mM | Aspergillus niger | |
K+ | about 115% activity at 1 mM | Aspergillus niger | |
Li+ | about 110% activity at 1 mM | Aspergillus niger | |
Mg2+ | about 120% activity at 1 mM | Aspergillus niger | |
Na+ | about 120% activity at 1 mM | Aspergillus niger | |
Zn2+ | about 115% activity at 1 mM | Aspergillus niger |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | A0A0B4UL66 | - |
- |
Aspergillus niger SC323 | A0A0B4UL66 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and ENrich SEC 650 gel filtration | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pectin + H2O | - |
Aspergillus niger | galacturonic acid + digalacturonic acid + trigalacturonic acid | - |
? | |
pectin + H2O | - |
Aspergillus niger SC323 | galacturonic acid + digalacturonic acid + trigalacturonic acid | - |
? | |
polygalacturonate + H2O | - |
Aspergillus niger | oligogalacturonates | - |
? | |
polygalacturonate + H2O | - |
Aspergillus niger SC323 | oligogalacturonates | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Aspergillus niger |
? | x * 38800, calculated from amino acid sequence | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
endo-pgaA | - |
Aspergillus niger |
endo-polygalacturonase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Aspergillus niger |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | more than 60% activity between 30 and 60°C | Aspergillus niger |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 70 | the enzyme retains more than 50% of its activity after incubation at 35-70°C for 60 min, but no activity remains after incubation at 70°C for 2 h | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | more than 60% activity between pH 3.0 and 6.0 | Aspergillus niger |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 6 | the enzyme shows good stability within the pH range of 3.0-6.0 for different incubation times and over 65% of activity is retained | Aspergillus niger |