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Literature summary for 3.2.1.15 extracted from
- Identification of an acidic endo-polygalacturonase from Penicillium oxalicum CZ1028 and its broad use in major tropical and subtropical fruit juices production (2017), J. Biosci. Bioeng., 123, 665-672 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | the enzyme significantly reduces the viscosities and improves the yields of fruit juices from banana, plantain, papaya, pitaya and mango | Penicillium oxalicum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Penicillium oxalicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 19.7% residual activity at 2 mM | Penicillium oxalicum |  |
| Mn2+ | 56.9% residual activity at 2 mM | Penicillium oxalicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 107.7 activity at 1 mM | Penicillium oxalicum | |
| additional information | the enzyme is not siginificantly influenced by Ba2+, Ca2+, Fe2+, K+, Mg2+, Na+, and Zn2+ | Penicillium oxalicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium oxalicum | A0A1B1MQM8 | - |
- |
| Penicillium oxalicum CZ1028 | A0A1B1MQM8 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Superdex 75 gel filtration | Penicillium oxalicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| apple pectin + H2O | - |
Penicillium oxalicum | ? | - |
? | |
| apple pectin + H2O | - |
Penicillium oxalicum CZ1028 | ? | - |
? | |
| citrus pectin + H2O | - |
Penicillium oxalicum | ? | - |
? | |
| citrus pectin + H2O | - |
Penicillium oxalicum CZ1028 | ? | - |
? | |
| polygalacturonate + H2O | highest activity | Penicillium oxalicum | oligogalacturonates | - |
? | |
| polygalacturonate + H2O | highest activity | Penicillium oxalicum CZ1028 | oligogalacturonates | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Penicillium oxalicum |
| ? | x * 37320, calculated from amino acid sequence | Penicillium oxalicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-PG | - |
Penicillium oxalicum |
| endo-polygalacturonase | - |
Penicillium oxalicum |
| EnPG28A | - |
Penicillium oxalicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Penicillium oxalicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 60 | more than 50% activity between 40 and 60°C, less than 20% activity at 65°C and above | Penicillium oxalicum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
the enzyme retains more than 90% of the initial activity after incubation at 45°C for 1 h | Penicillium oxalicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Penicillium oxalicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6 | more than 50% activity between pH 4.5 and 6.0 | Penicillium oxalicum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | the enzyme retains more than 80% enzyme activity at pH 3.0-10.0 when it is incubated at 25°C for 1 h, while it retains more than 80% enzyme activity at pH 4.0-6.0 when it is incubated at 25°C for 24 h | Penicillium oxalicum |
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