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Literature summary for 3.2.1.15 extracted from

  • Sassi, A.H.; Tounsi, H.; Trigui-Lahiani, H.; Bouzouita, R.; Romdhane, Z.B.; Gargouri, A.
    A low-temperature polygalacturonase from P. occitanis characterization and application in juice clarification (2016), Int. J. Biol. Macromol., 91, 158-164 .
    View publication on PubMed
Search for more literature for 3.2.1.15

Activating Compound

Activating Compound Comment Organism Structure
Urea 122% activity at 5 mM Penicillium sp. 'occitanis'

Application

Application Comment Organism
food industry the enzyme is used for juice clarification of pear, banana and citrus Penicillium sp. 'occitanis'

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ complete inhibition at 2 mM Penicillium sp. 'occitanis'
Ca2+ 64.9% residual activity at 2 mM Penicillium sp. 'occitanis'
Co2+ 0.31% residual activity at 2 mM Penicillium sp. 'occitanis'
Cu2+ 2.48% residual activity at 2 mM Penicillium sp. 'occitanis'
diethyl dicarbonate complete inhibition at 0.1% (w/v) Penicillium sp. 'occitanis'
EDTA 80% residual activity at 2 mM Penicillium sp. 'occitanis'
Fe2+ 16.45% residual activity at 2 mM Penicillium sp. 'occitanis'
K+ 87.5% residual activity at 2 mM Penicillium sp. 'occitanis'
Mn2+ complete inhibition at 1 mM Penicillium sp. 'occitanis'
additional information iodoacetamide, iodoacetate, dithiothreitol, 2-mercaptoethanol and Triton-X100 have no significant effect on enzyme activity Penicillium sp. 'occitanis'
Na+ 84.4% residual activity at 2 mM Penicillium sp. 'occitanis'
SDS 70.4% residual activity at 0.5% (w/v) Penicillium sp. 'occitanis'

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 132.9% activity at 1 mM Penicillium sp. 'occitanis'

Organism

Organism UniProt Comment Textmining
Penicillium sp. 'occitanis'
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion exchange column chromatography Penicillium sp. 'occitanis'

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
 Penicillium sp. 'occitanis' ?
-
 ?
polygalacturonate + H2O
-
 Penicillium sp. 'occitanis' oligogalacturonic acid + digalacturonic acid + trigalacturonic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 42000, SDS-PAGE Penicillium sp. 'occitanis'

Synonyms

Synonyms Comment Organism
endo-polygalacturonase
-
 Penicillium sp. 'occitanis'
PG2
-
 Penicillium sp. 'occitanis'
PGase
-
 Penicillium sp. 'occitanis'

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Penicillium sp. 'occitanis'

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 50 about 40% activity at 25°C, about 70% activity at 30°C, 100% activity at 35°C, about 80% activity at 40°C, about 40% activity at 50°C Penicillium sp. 'occitanis'

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 50 the enzyme retains about 90%, 80%, 75% and 10% activity after 7 h at 30°C, 35°C, 40°C, and 50°C, respectively Penicillium sp. 'occitanis'

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Penicillium sp. 'occitanis'

pH Range

pH Minimum pH Maximum Comment Organism
4 9 about 5% activity at pH 4.0, about 70% activity at pH 5.0, 100% activity at pH 6.0, about 85% activity at pH 7.0, about 50% activity at pH 8.0, about 5% activity at pH 9.0 Penicillium sp. 'occitanis'

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 the enzyme retains more than 80% activity after 24 h incubation at pH 4.0-9.0 Penicillium sp. 'occitanis'