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Literature summary for 3.2.1.15 extracted from

  • Hamada, S.; Toda, K.; Ogawa, S.; Kubota, K.; Miyairi, K.
    Characterization of the effects of C-terminal pro-sequence on self-inactivation of Stereum purpureum endopolygalacturonase I (2015), FEMS Microbiol. Lett., 362, fnv134 .
    View publication on PubMed
Search for more literature for 3.2.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta-gami 2 cells Chondrostereum purpureum

Protein Variants

Protein Variants Comment Organism
A365P the mutant shows about 70% of wild type activity Chondrostereum purpureum
D395N the mutant shows about 10% of wild type activity Chondrostereum purpureum
E364Q the mutant shows about 20% of wild type activity Chondrostereum purpureum
E364Q/E366Q the mutant shows about 60% of wild type activity Chondrostereum purpureum
H150A the mutant shows about 20% of wild type activity Chondrostereum purpureum
H195A inactive Chondrostereum purpureum
H98A inactive Chondrostereum purpureum
K205A inactive Chondrostereum purpureum
K228A inactive Chondrostereum purpureum
K253A the mutant shows about 50% of wild type activity Chondrostereum purpureum
K318A inactive Chondrostereum purpureum
K88A the mutant shows about 1% of wild type activity Chondrostereum purpureum
P339A the mutant shows about 5% of wild type activity Chondrostereum purpureum
P348A the mutant shows about 80% of wild type activity Chondrostereum purpureum
P352A the mutant shows about 35% of wild type activity Chondrostereum purpureum
P355A the mutant shows about 30% of wild type activity Chondrostereum purpureum
P358A the mutant shows about 7% of wild type activity Chondrostereum purpureum
R220A the mutant shows about 10% of wild type activity Chondrostereum purpureum
R226A inactive Chondrostereum purpureum

Organism

Organism UniProt Comment Textmining
Chondrostereum purpureum P79074
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and DE52-cellulose column chromatography Chondrostereum purpureum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.33
-
 crude extract, at pH 4.5 and 30°C Chondrostereum purpureum
435
-
 after 187fold purification, at pH 4.5 and 30°C Chondrostereum purpureum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
 Chondrostereum purpureum ?
-
 ?
polygalacturonate + H2O
-
 Chondrostereum purpureum oligogalacturonates
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Chondrostereum purpureum

Synonyms

Synonyms Comment Organism
EndoPG I
-
 Chondrostereum purpureum
endopolygalacturonase I
-
 Chondrostereum purpureum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Chondrostereum purpureum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 about 35% activity at 40°C, about 75% activity at 50°C, 100% activity at 60°C, about 60% activity at 70°C, about 45% activity at 80°C Chondrostereum purpureum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30 60 the enzyme activity remains stable (more than 90% activity) after 30 min between 30 and 60°C Chondrostereum purpureum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.4
-
 Pectin pro-enzyme, at pH 4.5 and 30°C Chondrostereum purpureum
20.2
-
 Pectin pro-enzyme, at pH 4.0 and 30°C Chondrostereum purpureum
230
-
 Pectin mature enzyme, at pH 4.5 and 30°C Chondrostereum purpureum
334
-
 Polygalacturonate pro-enzyme, at pH 4.5 and 30°C Chondrostereum purpureum
996
-
 Polygalacturonate mature enzyme, at pH 4.5 and 30°C Chondrostereum purpureum
1670
-
 Polygalacturonate pro-enzyme, at pH 4.0 and 30°C Chondrostereum purpureum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
-
 Chondrostereum purpureum

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 the enzyme activity remains stable after 16 h between pH 3.0 and 7.0 Chondrostereum purpureum