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Literature summary for 3.2.1.15 extracted from
- Kinetic properties of Rhizopus oryzae RPG1 endo-polygalacturonase hydrolyzing galacturonic acid oligomers (2016), Biocatal. Agricult. Biotechnol., 5, 11-16 .
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Digalacturonate | competitive inhibition | Rhizopus arrhizus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0099 | - |
hexagalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
| 0.0365 | - |
pentagalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
| 0.0681 | - |
tetragalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
| 0.149 | - |
trigalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus arrhizus | Q5W9U0 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hexagalacturonate + H2O | - |
Rhizopus arrhizus | trigalacturonate | - |
? | |
| pentagalacturonate + H2O | - |
Rhizopus arrhizus | trigalacturonate + digalacturonate + tetragalacturonate + galacturonate | - |
? | |
| polygalacturonate + H2O | - |
Rhizopus arrhizus | trigalacturonate | - |
? | |
| tetragalacturonate + H2O | - |
Rhizopus arrhizus | trigalacturonate + galacturonate | - |
? | |
| trigalacturonate + H2O | - |
Rhizopus arrhizus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-polygalacturonase | - |
Rhizopus arrhizus |
| endoPG | - |
Rhizopus arrhizus |
| RPG1 | - |
Rhizopus arrhizus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.6 | - |
trigalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
| 27.4 | - |
tetragalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
| 35.8 | - |
pentagalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
| 45.7 | - |
hexagalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0767 | - |
Digalacturonate | at pH 4.0 and 30°C | Rhizopus arrhizus | |
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