Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | activates by 24.5% | Achaetomium sp. Xz-8 |
Application | Comment | Organism |
---|---|---|
food industry | the enzyme reduces the viscosity of papaya juice by 17.6% and increases its transmittance by 59.1%. Its favourable enzymatic properties make the enzyme attractive for potential applications in the juice industry | Achaetomium sp. Xz-8 |
Cloned (Comment) | Organism |
---|---|
gene pg I, DNA and amino acid sequence determination and analysis, overexpression in Pichia pastoris in fed-batch fermentation | Achaetomium sp. Xz-8 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | over 50% inhibition | Achaetomium sp. Xz-8 | |
Ca2+ | over 50% inhibition | Achaetomium sp. Xz-8 | |
Cr3+ | below 40% inhibition | Achaetomium sp. Xz-8 | |
Cu2+ | below 40% inhibition | Achaetomium sp. Xz-8 | |
EDTA | below 40% inhibition | Achaetomium sp. Xz-8 | |
Mg2+ | below 40% inhibition | Achaetomium sp. Xz-8 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35400 | - |
x * 35400, about, sequence calculation, x * 36900, about, mass spectrometry | Achaetomium sp. Xz-8 |
36900 | - |
x * 35400, about, sequence calculation, x * 36900, about, mass spectrometry | Achaetomium sp. Xz-8 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Achaetomium sp. Xz-8 | S5MK25 | isolated from a desert sand sample of Ningxia, China, gene pg I | - |
Achaetomium sp. Xz-8 Xz8 | S5MK25 | isolated from a desert sand sample of Ningxia, China, gene pg I | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | N-glycosylation | Achaetomium sp. Xz-8 |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris strain GS115 by ultrafiltration, gel filtration, and anion exchange chromatography, followed by deglycosylation of the proteins by endo-beta-N-acetylglucosaminidase H | Achaetomium sp. Xz-8 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28120 | - |
purified recombinant enzyme, pH 6.0, 45°C | Achaetomium sp. Xz-8 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | endo-PG I is a polygalacturonase of endo-type | Achaetomium sp. Xz-8 | ? | - |
? | |
additional information | endo-PG I is a polygalacturonase of endo-type | Achaetomium sp. Xz-8 Xz8 | ? | - |
? | |
pectin + H2O | citrus pectin | Achaetomium sp. Xz-8 | ? | - |
? | |
pectin + H2O | citrus pectin | Achaetomium sp. Xz-8 Xz8 | ? | - |
? | |
polygalacturonic acid + H2O | - |
Achaetomium sp. Xz-8 | digalacturonic acid + galacturonic acid + trigalacturonic acid | - |
? | |
polygalacturonic acid + H2O | - |
Achaetomium sp. Xz-8 Xz8 | digalacturonic acid + galacturonic acid + trigalacturonic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35400, about, sequence calculation, x * 36900, about, mass spectrometry | Achaetomium sp. Xz-8 |
Synonyms | Comment | Organism |
---|---|---|
endo-PG I | - |
Achaetomium sp. Xz-8 |
endo-polygalacturonase | - |
Achaetomium sp. Xz-8 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Achaetomium sp. Xz-8 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 80 | activity range | Achaetomium sp. Xz-8 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | purified recombinant enzyme, pH 6.0, 1 h without substrate, completely stable | Achaetomium sp. Xz-8 |
55 | - |
purified recombinant enzyme, pH 6.0, 1 h without substrate,loss of 70% activity | Achaetomium sp. Xz-8 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Achaetomium sp. Xz-8 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | 40% of maximal activity within this range | Achaetomium sp. Xz-8 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8 | purified recombinant enzyme, 37°C, 1 h without substrate, over 75% activity remaining | Achaetomium sp. Xz-8 |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the family 28 of glycosyl hydrolases | Achaetomium sp. Xz-8 |
physiological function | the enzyme reduces the viscosity of papaya juice by 17.6% and increases its transmittance by 59.1%. The enzyme works synergistically with pectin methylesterase | Achaetomium sp. Xz-8 |