Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant EndoPG Is are randomly glycosylated at four N-glycosylation sites | Chondrostereum purpureum |
Cloned (Comment) | Organism |
---|---|
ligated into vector pPICZalpha A, transformed into competent cells of Escherichia coli JM109. Expressed in Pichia pastoris GS115 strain | Chondrostereum purpureum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36430 | 36750 | MALDI-TOF-MS analysis | Chondrostereum purpureum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chondrostereum purpureum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | recombinant EndoPG is with differing numbers of sugar chains (1-4), purified by ammonium sulfate precipitation and cation exchange chromatography, between 22.4-32.6fold | Chondrostereum purpureum |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.7 | - |
culture supernatant of EndoPG I | Chondrostereum purpureum |
307 | - |
22.4fold purified recombinant EndoPG I with four sugar chains | Chondrostereum purpureum |
408 | - |
29.8fold purified recombinant EndoPG I with one sugar chain | Chondrostereum purpureum |
418 | - |
30.5fold purified recombinant EndoPG I with three sugar chains | Chondrostereum purpureum |
447 | - |
32.6fold purified recombinant EndoPG I with two sugar chains | Chondrostereum purpureum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonic acid + H2O | - |
Chondrostereum purpureum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
EndoPG I | - |
Chondrostereum purpureum |
endopolygalacturonase I | - |
Chondrostereum purpureum |