Literature summary for 3.2.1.15 extracted from

Hsiao, Y.M.; Zheng, M.H.; Hu, R.M.; Yang, T.C.; Tseng, Y.H.
Regulation of the pehA gene encoding the major polygalacturonase of Xanthomonas campestris by Clp and RpfF (2008), Microbiology, 154, 705-713.

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	pehA transcription is greatly induced by polygalacturonic acid and positively regulated by Clp (cAMP receptor protein-like protein) and RpfF (an enoyl-CoA hydratase homologue which is required for the synthesis of cis-11-methyl-2-dodecenoic acid, a low-molecular-mass diffusible signal factor)	Xanthomonas campestris pv. campestris

Application

Application	Comment	Organism
additional information	PehA is the major but not the sole polygalacturonase, it plays a minor role in Xanthomonas campestris virulence	Xanthomonas campestris pv. campestris

Cloned(Commentary)

Cloned (Comment)	Organism
1620 bp DNA fragment encompassing the upstream 218 bp plus the entire coding region of the Xc17 pehA into the PstI-XbaI sites of the broad-host-range vector pRK415. 1376 bp NdeI-XhoI fragment containing the Xc17 pehA gene cloned in the Escherichia coli vector pOK12, giving pOKpeh. A Gmr cartridge from pUCGM inserted into the unique HincII site within the pOKpeh insert. Resultant plasmid, pOKpehG, electroporated into strain Xc17 allowing for double crossover. PpehA-lacZ transcriptional fusion constructs generated by cloning PCR fragments into the broad-host-range promoter-probing vector pFY13-9, which uses lacZ as the reporter	Xanthomonas campestris pv. campestris

Cloned (Comment)

Organism

1620 bp DNA fragment encompassing the upstream 218 bp plus the entire coding region of the Xc17 pehA into the PstI-XbaI sites of the broad-host-range vector pRK415. 1376 bp NdeI-XhoI fragment containing the Xc17 pehA gene cloned in the Escherichia coli vector pOK12, giving pOKpeh. A Gmr cartridge from pUCGM inserted into the unique HincII site within the pOKpeh insert. Resultant plasmid, pOKpehG, electroporated into strain Xc17 allowing for double crossover. PpehA-lacZ transcriptional fusion constructs generated by cloning PCR fragments into the broad-host-range promoter-probing vector pFY13-9, which uses lacZ as the reporter

Xanthomonas campestris pv. campestris

Protein Variants

Protein Variants	Comment	Organism
additional information	in mutant MH172 with cloned pehA, MH172(pRKpeh), wild-type-level polygalacturonase activity is restored	Xanthomonas campestris pv. campestris

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	expression of pehA is subject to catabolite repression, which is independent of Clp and RpfF, and it is repressed under conditions of oxygen limitation or nitrogen starvation	Xanthomonas campestris pv. campestris

Organism

Organism	UniProt	Comment	Textmining
Xanthomonas campestris pv. campestris	-	-	-
Xanthomonas campestris pv. campestris Xc17	-	-	-

Synonyms

Synonyms	Comment	Organism
PehA	-	Xanthomonas campestris pv. campestris