Cloned (Comment) | Organism |
---|---|
into plasmid pET22b and transformed into Escherichia coli strain DH5alpha, expressed in Escherichia coli BL21(DE3) cells | Burkholderia cepacia |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Burkholderia cepacia | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration, wild-type | Burkholderia cepacia |
45000 | - |
gel filtration, recombinant protein in solution | Burkholderia cepacia |
47000 | - |
1 * 47000, purified enzyme, SDS-PAGE | Burkholderia cepacia |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia cepacia | P94293 | strain ATCC 25416 | - |
Purification (Comment) | Organism |
---|---|
to homogeneity, 5.8fold by cation exchange chromatography and gel filtration | Burkholderia cepacia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.6 | - |
supernatant | Burkholderia cepacia |
21.1 | - |
5.8fold purified enzyme | Burkholderia cepacia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonic acid + H2O | - |
Burkholderia cepacia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 47000, purified enzyme, SDS-PAGE | Burkholderia cepacia |
Synonyms | Comment | Organism |
---|---|---|
endo-polygalacturonase | - |
Burkholderia cepacia |
endoPG | - |
Burkholderia cepacia |
PehA | - |
Burkholderia cepacia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
exhibits a relatively flat activity profile in a broad temperature interval, displaying an optimal activity in the temperature interval of 40-55°C | Burkholderia cepacia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme is thermolabile, being completely inactivated if kept at 50°C for 2 h | Burkholderia cepacia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Burkholderia cepacia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Burkholderia cepacia | purified enzyme under native conditions | - |
7.8 |