Any feedback?
Literature summary for 3.2.1.15 extracted from
- Isolation and characterization of a tomato non-specific lipid transfer protein involved in polygalacturonase-mediated pectin degradation (2007), J. Exp. Bot., 58, 1151-1160.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | a 8 kDa protein (ACT) from the PG1 protein complex is capable of converting the gene product PG2 into a more active and heat-stable form, which increases PG-mediated pectin degradation in vitro and stimulates PG-mediated tissue breakdown in planta. Addition of the 8 kDa protein to tomato PG2 causes a 4fold to 5fold increase in activity | Solanum lycopersicum | |
| additional information | apple or peach lipid transfer proteins significantly increase PG activity (more than 2fold) and heat stability | Aspergillus niger |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
1 * 45000, SDS-PAGE, PG2 | Solanum lycopersicum |
| 100000 | - |
gel filtration, PG1 | Solanum lycopersicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus niger | - |
- |
- |
| Solanum lycopersicum | - |
cultivar Counter | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus niger |
| by gel filtration | Solanum lycopersicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit | high activity of PG2 in ripe tomato fruit pericarp tissue | Solanum lycopersicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Aspergillus niger | galacturonic acid oligomers | - |
? |  |
| pectin + H2O | - |
Solanum lycopersicum | galacturonic acid oligomers | - |
? | |
| polygalacturonic acid + H2O | - |
Aspergillus niger | ? | - |
? | |
| polygalacturonic acid + H2O | - |
Solanum lycopersicum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 1 * 45000, SDS-PAGE, PG2 | Solanum lycopersicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PG1 | - |
Solanum lycopersicum |
| PG2 | - |
Solanum lycopersicum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
