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Literature summary for 3.2.1.15 extracted from
- Production, characterization and application of a thermostable polygalacturonase of a thermophilic mould Sporotrichum thermophils Apinis (2004), Biores. Technol., 94, 239-243.
Application
| Application | Comment | Organism |
|---|---|---|
| nutrition | potential application of the enzyme in fruit juice extraction | Thermothelomyces heterothallicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Thermothelomyces heterothallicus |  |
| Ca2+ | 5 mM | Thermothelomyces heterothallicus | |
| Cu2+ | 5 mM | Thermothelomyces heterothallicus | |
| Mg2+ | strong inhibition | Thermothelomyces heterothallicus | |
| SDS | - |
Thermothelomyces heterothallicus | |
| Triton X-100 | slight inhibition | Thermothelomyces heterothallicus | |
| Tween 80 | slight inhibition | Thermothelomyces heterothallicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | the KM-value for pectin is 0.416 mg/ml | Thermothelomyces heterothallicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 1 mM, stimulates | Thermothelomyces heterothallicus | |
| Cu2+ | 1 mM, stimulates | Thermothelomyces heterothallicus | |
| Fe2+ | 1 mM and 5 mM, stimulates | Thermothelomyces heterothallicus | |
| Mn2+ | 1 mM and 5 mM, stimulates | Thermothelomyces heterothallicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces heterothallicus | - |
Apinis | - |
| Thermothelomyces heterothallicus Apinis | - |
Apinis | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermothelomyces heterothallicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectin + H2O | - |
Thermothelomyces heterothallicus | ? | - |
? | |
| pectin + H2O | - |
Thermothelomyces heterothallicus Apinis | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Thermothelomyces heterothallicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 70 | 50°C: about 55% of maximal activity, 70°C: about 50% of maximal activity | Thermothelomyces heterothallicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
t1/2: 4 h | Thermothelomyces heterothallicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Thermothelomyces heterothallicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | pH 4.0: about 50% of maximal activity, pH 8.0: about 50% of maximal activity | Thermothelomyces heterothallicus |
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Release 2023.1 (January 2023)
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