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Literature summary for 3.2.1.15 extracted from
- Pectinase production by Neurospora crassa: purification and biochemical characterization of extracellular polygalacturonase activity (1991), J. Gen. Microbiol., 137, 1815-1823.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| polypectate | substrate inhibition at high concentrations | Neurospora crassa |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Neurospora crassa |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 36600 | - |
gel filtration | Neurospora crassa |
| 37000 | - |
1 * 37000, SDS-PAGE | Neurospora crassa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Neurospora crassa | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 38% carbohydrate | Neurospora crassa |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Neurospora crassa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, good stability | Neurospora crassa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pectic acid + H2O | - |
Neurospora crassa | galacturonic acid oligomers | - |
? | |
| pectin + H2O | - |
Neurospora crassa | ? | - |
? | |
| polygalacturonate + H2O | - |
Neurospora crassa | oligogalacturonates | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 37000, SDS-PAGE | Neurospora crassa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Neurospora crassa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
half-life: 4 min | Neurospora crassa |
| 60 | - |
half-life: 1.2 min | Neurospora crassa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Neurospora crassa |
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Release 2023.1 (January 2023)
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