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Literature summary for 3.2.1.15 extracted from

  • Urbanek, H.; Zalewska-Sobczak, J.A.
    Polygalacturonase of Botrytis cinerea E-200 Pers (1975), Biochim. Biophys. Acta, 377, 402-409.
    View publication on PubMed
Search for more literature for 3.2.1.15

Inhibitors

Inhibitors Comment Organism Structure
PCMB
-
 Botrytis cinerea
Tetranitromethane
-
 Botrytis cinerea
Urea
-
 Botrytis cinerea

Organism

Organism UniProt Comment Textmining
Botrytis cinerea
-
 E-200 PERS
-
Botrytis cinerea E-200 PERS
-
 E-200 PERS
-

Purification (Commentary)

Purification (Comment) Organism
-
 Botrytis cinerea

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Botrytis cinerea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2049
-
-
 Botrytis cinerea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O at 14% of the activity with polypectate Botrytis cinerea D-galacturonic acid
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O at 14% of the activity with polypectate Botrytis cinerea E-200 PERS D-galacturonic acid
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O 52% of the activity with polypectate Botrytis cinerea digalacturonic acid + galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O 52% of the activity with polypectate Botrytis cinerea E-200 PERS digalacturonic acid + galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O 79% of the activity with polypectate Botrytis cinerea alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + D-galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O 79% of the activity with polypectate Botrytis cinerea E-200 PERS alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + D-galacturonate
-
 ?
polygalacturonate + H2O
-
 Botrytis cinerea oligogalacturonates
-
 ?
polygalacturonate + H2O
-
 Botrytis cinerea E-200 PERS oligogalacturonates
-
 ?
polypectate + H2O oligogalacturonides gradually disappear with the reaction time, so that monogalacturonic acid remains as the only product of the hydrolysis Botrytis cinerea ?
-
 ?
polypectate + H2O oligogalacturonides gradually disappear with the reaction time, so that monogalacturonic acid remains as the only product of the hydrolysis Botrytis cinerea E-200 PERS ?
-
 ?