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Literature summary for 3.2.1.15 extracted from
- Production, purification and partial characterization of an endopolygalacturonase from Cryptococcus albidus var. albidus (1985), Antonie van Leeuwenhoek, 51, 139-150.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | 5 mM, complete inhibition | Naganishia albida |  |
| Hg2+ | - |
Naganishia albida | |
| Pb2+ | 5 mM, complete inhibition | Naganishia albida | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Naganishia albida |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Naganishia albida | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
gel filtration | Naganishia albida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Naganishia albida | - |
var. albidus | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Naganishia albida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Naganishia albida | oligogalacturonates | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 41000, SDS-PAGE | Naganishia albida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Naganishia albida |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 50 | about 50% of maximal activity at 25°C and 50°C | Naganishia albida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
stable up to | Naganishia albida |
| 55 | - |
10 min, complete inactivation | Naganishia albida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
- |
Naganishia albida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 4.5 | pH 3.5: about 60% of maximal activity, 50°C: about 50% of maximal activity | Naganishia albida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | stable | Naganishia albida |
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Release 2023.1 (January 2023)
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