Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.149 extracted from

  • Mizutani, M.; Nakanishi, H.; Ema, J.; Ma, S.J.; Noguchi, E.; Inohara-Ochiai, M.; Fukuchi-Mizutani, M.; Nakao, M.; Sakata, K.
    Cloning of beta-primeverosidase from tea leaves, a key enzyme in tea aroma formation (2002), Plant Physiol., 130, 2164-2176.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.149

Application

Application Comment Organism
nutrition beta-primeverosidase is a key enzyme in tea aroma formation Camellia sinensis

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Camellia sinensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 x * 61000, SDS-PAGE Camellia sinensis

Organism

Organism UniProt Comment Textmining
Camellia sinensis Q7X9A9 var sinensis cv Yabukita
-

Purification (Commentary)

Purification (Comment) Organism
-
 Camellia sinensis

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Camellia sinensis
-
stem
-
 Camellia sinensis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
 purified enzyme Camellia sinensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-phenylethyl beta-primeveroside + H2O pH 6.0, 37°C, 100% activity Camellia sinensis primeverose + 2-phenylethanol
-
 ?
additional information no activity with p-nitrophenol beta-D-xyloside and 2-phenylethyl beta-D-glucoside Camellia sinensis ?
-
 ?
p-nitrophenyl beta-D-glucoside + H2O pH 6.0, 37°C, 0.5% activity (purified from tea), 0.3% activity (recombinant enzyme) Camellia sinensis p-nitrophenol + D-glucose
-
 ?
p-nitrophenyl beta-primeveroside + H2O pH 6.0, 37°C, 48% activity (purified from tea), 62% activity (recombinant enzyme) Camellia sinensis primeverose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Camellia sinensis

Synonyms

Synonyms Comment Organism
beta-primeverosidase
-
 Camellia sinensis