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Literature summary for 3.2.1.149 extracted from

  • Tsuruhami, K.; Mori, S.; Amarume, S.; Saruwatari, S.; Murata, T.; Hirakake, J.; Sakata, K.; Usui, T.
    Isolation and Characterization of a beta-primeverosidase-like enzyme from Penicillium multicolor (2006), Biosci. Biotechnol. Biochem., 70, 691-698.
    View publication on PubMed
Search for more literature for 3.2.1.149

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 eugenyl beta-primeveroside pH 5.5, 40°C Penicillium multicolor
12
-
 p-nitrophenyl beta-primeveroside pH 5.5, 40°C Penicillium multicolor
21
-
 p-nitrophenyl beta-gentiobioside pH 5.5, 40°C Penicillium multicolor
47
-
 p-nitrophenyl beta-D-glucopyranoside pH 5.5, 40°C Penicillium multicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 monomer, SDS-PAGE, gel filtration Penicillium multicolor

Organism

Organism UniProt Comment Textmining
Penicillium multicolor
-
 IAM7153
-
Penicillium multicolor IAM7153
-
 IAM7153
-

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium multicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
 purified enzyme Penicillium multicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Z)-3-hexenyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor (Z)-3-hexenol + primeverose
-
 ?
(Z)-3-hexenyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor IAM7153 (Z)-3-hexenol + primeverose
-
 ?
2-phenylethyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor 2-phenylethanol + primeverose
-
 ?
2-phenylethyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor IAM7153 2-phenylethanol + primeverose
-
 ?
4-nitrophenyl beta-glucopyranoside + H2O pH 5.5, 40°C, 15% hydrolysis Penicillium multicolor 4-nitrophenol + D-glucopyranose
-
 ?
benzyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor phenylmethanol + primeverose
-
 ?
benzyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor IAM7153 phenylmethanol + primeverose
-
 ?
eugenyl beta-primeveroside + H2O pH 5.5, 40°C, 2.5% hydrolysis Penicillium multicolor eugenol + primeverose
-
 ?
eugenyl beta-primeveroside + H2O pH 5.5, 40°C, 2.5% hydrolysis Penicillium multicolor IAM7153 eugenol + primeverose
-
 ?
furcatin + H2O pH 5.5, 40°C, 1.0% hydrolysis Penicillium multicolor 4-allyl-phenol + ?
-
 ?
furcatin + H2O pH 5.5, 40°C, 1.0% hydrolysis Penicillium multicolor IAM7153 4-allyl-phenol + ?
-
 ?
geranyl beta-primeveroside + H2O pH 5.5, 40°C, 0.1% hydrolysis Penicillium multicolor geraniol + primeverose
-
 ?
p-nitrophenyl beta-gentiobioside + H2O pH 5.5, 40°C, 2.5% hydrolysis Penicillium multicolor p-nitrophenol + D-gentiobiose
-
 ?
p-nitrophenyl beta-primeveroside + H2O pH 5.5, 40°C, 100% hydrolysis Penicillium multicolor primeverose + p-nitrophenol
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 50000, gel filtration, SDS-PAGE Penicillium multicolor

Synonyms

Synonyms Comment Organism
beta-primeverosidase
-
 Penicillium multicolor

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Penicillium multicolor

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60 near optimum activities observed Penicillium multicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
 Penicillium multicolor