BRENDA - Enzyme Database
show all sequences of 3.2.1.149

Characterization of beta-primeverosidase, being concerned with alcoholic aroma formation in tea leaves to be processed into black tea, and preliminary observations on its substrate specificity

Ijima, Y.; Ogawa, K.; Watanabe, N.; Usui, T.; Ohnishi-Kameyama, M.; Nagata, T.; Sakata, K.; J. Agric. Food Chem. 46, 1712-1718 (1998)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60170
-
var. sinensis cv. Shuixian, MALDI-TOF-MS
Camellia sinensis
60310
-
var. assamica, MALDI-TOFMS
Camellia sinensis
60480
-
var. sinensis cv. Yabukita, MALDI-TOF-MS
Camellia sinensis
61000
-
SDS-PAGE
Camellia sinensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-linalyl beta-vicianoside + H2O
Camellia sinensis
-
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
-
Camellia sinensis
?
eugenyl beta-primeveroside + H2O
Camellia sinensis
i.e. eugenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
eugenol + primeverose
-
-
?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
Camellia sinensis
-
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
-
Camellia sinensis
?
Organism
Organism
UniProt
Commentary
Textmining
Camellia sinensis
-
-
-
Camellia sinensis
-
var. sinensis cv. Yabukita
-
Camellia sinensis
-
var. sinensis cv. Shuixian
-
Camellia sinensis
-
var. assamica
-
Purification (Commentary)
Purification (Commentary)
Organism
var. assamica
Camellia sinensis
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
fresh, non-fermented
Camellia sinensis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.9
0.99
-
Camellia sinensis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
(R)-linalyl beta-vicianoside + H2O
-
288803
Camellia sinensis
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
-
288803
Camellia sinensis
?
4-nitrophenyl beta-primeveroside + H2O
i.e. 4-nitrophenyl-6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
288803
Camellia sinensis
4-nitrophenol + primeverose
-
288803
Camellia sinensis
?
benzyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
-
288803
Camellia sinensis
benzyl alcohol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
-
-
-
?
benzyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
-
288803
Camellia sinensis
benzyl alcohol + beta-D-apiofuranosyl-(1->6)-D-glucose
-
-
-
?
eugenyl beta-primeveroside + H2O
i.e. eugenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
288803
Camellia sinensis
eugenol + primeverose
-
-
-
?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
-
288803
Camellia sinensis
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
-
288803
Camellia sinensis
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Camellia sinensis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
-
Camellia sinensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
60170
-
var. sinensis cv. Shuixian, MALDI-TOF-MS
Camellia sinensis
60310
-
var. assamica, MALDI-TOFMS
Camellia sinensis
60480
-
var. sinensis cv. Yabukita, MALDI-TOF-MS
Camellia sinensis
61000
-
SDS-PAGE
Camellia sinensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
(R)-linalyl beta-vicianoside + H2O
Camellia sinensis
-
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
-
Camellia sinensis
?
eugenyl beta-primeveroside + H2O
Camellia sinensis
i.e. eugenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
eugenol + primeverose
-
-
?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
Camellia sinensis
-
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
-
Camellia sinensis
?
Purification (Commentary) (protein specific)
Commentary
Organism
var. assamica
Camellia sinensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
fresh, non-fermented
Camellia sinensis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.9
0.99
-
Camellia sinensis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
(R)-linalyl beta-vicianoside + H2O
-
288803
Camellia sinensis
linalol + 6-O-alpha-L-arabinopyranosyl-beta-D-glucopyranoside
-
288803
Camellia sinensis
?
4-nitrophenyl beta-primeveroside + H2O
i.e. 4-nitrophenyl-6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
288803
Camellia sinensis
4-nitrophenol + primeverose
-
288803
Camellia sinensis
?
benzyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
-
288803
Camellia sinensis
benzyl alcohol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
-
-
-
?
benzyl 6-O-beta-D-apiofuranosyl-beta-D-glucopyranoside + H2O
-
288803
Camellia sinensis
benzyl alcohol + beta-D-apiofuranosyl-(1->6)-D-glucose
-
-
-
?
eugenyl beta-primeveroside + H2O
i.e. eugenyl 6-O-beta-D-xylopyranosyl-beta-D-glucopyranose
288803
Camellia sinensis
eugenol + primeverose
-
-
-
?
geranyl 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside + H2O
-
288803
Camellia sinensis
geraniol + 6-O-alpha-L-arabinofuranosyl-beta-D-glucopyranoside
-
288803
Camellia sinensis
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Camellia sinensis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
-
-
Camellia sinensis
Other publictions for EC 3.2.1.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
754216
Sato
Enhancing monoterpene alcohol ...
Ipomoea batatas
J. Biosci. Bioeng.
125
218-223
2018
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
1
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-
-
-
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-
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2
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2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
753640
Zhou
Functional characterizations ...
Camellia sinensis
Food Res. Int.
96
206-214
2017
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
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-
-
-
-
-
-
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-
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-
1
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2
-
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-
-
2
-
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-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
753954
Gui
Does enzymatic hydrolysis of ...
Camellia sinensis
J. Agric. Food Chem.
63
6905-6914
2015
-
-
1
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
1
-
-
-
1
-
-
-
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-
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-
1
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-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
731973
Zhou
Occurrence of glycosidically c ...
Camellia sinensis
J. Agric. Food Chem.
62
8042-8050
2014
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
1
-
-
2
-
-
4
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
732136
Saino
Crystal structures of beta-pri ...
Camellia sinensis
J. Biol. Chem.
289
16826-16834
2014
-
-
1
1
-
-
2
-
-
-
-
1
-
1
-
1
1
-
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
2
1
-
-
-
-
1
-
-
1
1
-
1
-
-
5
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
678826
Saino
Expression and biochemical cha ...
Camellia sinensis
Biosci. Biotechnol. Biochem.
72
376-383
2008
-
-
1
-
-
-
3
-
-
-
1
-
-
3
-
-
1
-
-
-
2
-
4
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
3
2
-
-
-
1
-
-
-
-
1
-
-
2
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
664388
Tsuruhami
Isolation and Characterization ...
Penicillium multicolor, Penicillium multicolor IAM7153
Biosci. Biotechnol. Biochem.
70
691-698
2006
-
-
-
-
-
-
-
4
-
-
1
-
-
5
-
-
1
-
-
-
1
-
14
1
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
1
-
-
1
-
14
1
1
1
-
-
1
-
-
-
-
-
-
-
-
-
681660
Han
-
Toward a blueprint for beta -p ...
Camellia sinensis
J. Theor. Comput. Chem.
5
433-446
2006
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
2
-
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-
-
-
-
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-
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-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
666247
Sakata
Diglycoside-specific glycosida ...
Camellia sinensis
Methods Enzymol.
363
444-459
2003
-
-
2
-
-
-
-
2
-
-
4
-
-
3
-
-
1
-
-
-
4
-
9
1
1
1
-
2
2
1
-
2
-
-
2
-
-
-
2
-
-
-
-
-
-
-
2
-
-
4
-
-
-
-
1
-
-
4
-
9
1
1
-
2
2
1
-
2
2
-
-
-
-
-
-
664348
Yamamoto
Isolation and characterization ...
Aspergillus fumigatus, Aspergillus fumigatus AP-20
Biosci. Biotechnol. Biochem.
66
801-807
2002
-
-
-
-
-
-
-
3
-
-
1
-
-
2
-
-
1
-
-
-
1
-
13
1
1
1
1
-
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
1
-
-
1
-
13
1
1
1
-
-
1
1
-
1
-
-
-
-
-
-
666618
Mizutani
Cloning of beta-primeverosidas ...
Camellia sinensis
Plant Physiol.
130
2164-2176
2002
-
1
1
-
-
-
-
-
-
-
1
-
-
14
-
-
1
-
-
2
1
-
4
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
2
1
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
288806
Ma
Substrate specificity of beta- ...
Camellia sinensis
Biosci. Biotechnol. Biochem.
65
2719-2729
2001
-
-
-
-
-
-
-
4
-
-
-
3
-
12
-
-
1
-
-
1
-
-
11
-
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
3
-
-
-
1
-
1
-
-
11
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
288803
Ijima
Characterization of beta-prime ...
Camellia sinensis
J. Agric. Food Chem.
46
1712-1718
1998
-
-
-
-
-
-
-
-
-
-
4
3
-
4
-
-
1
-
-
1
1
-
6
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
3
-
-
-
1
-
1
1
-
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
288804
Ogawa
Purification of a beta-primeve ...
Camellia sinensis
J. Agric. Food Chem.
45
877-882
1997
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
1
-
-
-
-
1
1
1
-
1
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
-
-
1
1
1
-
1
1
1
-
-
-
-
-
-
-
288805
Guo
-
Studies on the aroma formation ...
Camellia sinensis
Biosci. Biotechnol. Biochem.
60
1810-1814
1996
-
-
-
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
1
5
-
5
-
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
5
-
5
-
1
-
1
-
1
-
1
-
-
-
-
-
-
-