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Literature summary for 3.2.1.147 extracted from

  • Fahey, J.W.; Holtzclaw, W.D.; Wehage, S.L.; Wade, K.L.; Stephenson, K.K.; Talalay, P.
    Sulforaphane bioavailability from glucoraphanin-rich broccoli control by active endogenous myrosinase (2015), PLoS ONE, 10, e0140963 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
pharmacology sulforaphane, the reactive isothiocyanate that potently inhibits neoplastic cellular processes and prevents a number of disease states in humans, is difficult to deliver in an enriched and stable form for purposes of direct human consumption. Evaluation of the bioavailability of sulforaphane, either by direct administration of glucoraphanin (a glucosinolate, or beta-thioglucoside-N-hydroxysulfate), or by co-administering glucoraphanin and the enzyme myrosinase to catalyze its conversion to sulforaphane at economic, reproducible and sustainable yields, overview. Following administration of glucoraphanin in a commercially prepared dietary supplement to a small number of human volunteers, the volunteers have equivalent output of sulforaphane metabolites in their urine to that which they produce when given an equimolar dose of glucoraphanin in a simple boiled and lyophilized extract of broccoli sprouts. Furthermore, when either broccoli sprouts or seeds are administered directly to subjects without prior extraction and consequent inactivation of endogenous myrosinase, regardless of the delivery matrix or dose, the sulforaphane in those preparations is 3 to 4fold more bioavailable than sulforaphane from glucoraphanin delivered without active plant myrosinase Brassica oleracea var. italica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucoraphanin + H2O Brassica oleracea var. italica
-
sulforaphane + D-glucose
-
?
sinigrin + H2O Brassica oleracea var. italica
-
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. italica A0A343IQS8
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
Brassica oleracea var. italica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucoraphanin + H2O
-
Brassica oleracea var. italica sulforaphane + D-glucose
-
?
sinigrin + H2O
-
Brassica oleracea var. italica (1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
?

Synonyms

Synonyms Comment Organism
myrosinase
-
Brassica oleracea var. italica

General Information

General Information Comment Organism
physiological function glucoraphanin from broccoli and its sprouts and seeds is a water soluble and relatively inert precursor of sulforaphane, the reactive isothiocyanate that is formed by the activity of myrosinase and potently inhibits neoplastic cellular processes. It prevents a number of disease states in humans Brassica oleracea var. italica