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Literature summary for 3.2.1.147 extracted from
- High pressure effects on myrosinase activity and glucosinolate preservation in seedlings of Brussels sprouts (2018), Food Chem., 245, 1212-1217 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | - |
Brassica oleracea var. gemmifera |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | increasing the pressure level (100-800 MPa) results in a decrease in the myrosinase activity in accordance with previous findings of the pressure effect on myrosinase from Brussels sprouts seedlings, dependent also on the temperature, modeling, overview. Enzyme inactivation at 800 MPa | Brassica oleracea var. gemmifera |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica oleracea var. gemmifera | Q1W0X8 | cv. Evesham special | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from powder of freeze-dried seedlings of Brussels sprouts, by solubilization, and concanavalin A affinity chromatography with elution by methyl-alpha-D-mannopyranoside | Brassica oleracea var. gemmifera |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Brassica oleracea var. gemmifera | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sinigrin + H2O | - |
Brassica oleracea var. gemmifera | (1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| myrosinase | - |
Brassica oleracea var. gemmifera |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Brassica oleracea var. gemmifera |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | highest activity at 30°C within this range | Brassica oleracea var. gemmifera |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
assay at | Brassica oleracea var. gemmifera |
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Release 2023.1 (January 2023)
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